Genetic effects of biopad® on fibroblast primary culture

A. Baj, G. A. Beltramini, M. Romano, D. Lauritano, R. M. Gaudio, A. Palmieri, F. Cura, A. B. Gianni

Research output: Contribution to journalArticle

Abstract

BIOPAD® is an ivory-white soft sponge, made exclusively of lyophilized type I native heterologous collagen extracted from horse flexor tendon, gelatine free, that keeps its native structure specific to the body's skin tissue. BIOPAD® is an active dressing, playing an active role in all stages of wound healing process, stimulating granulation tissue growth and enhancing regeneration tissues. It ensures balance between absorption and humidity at wound surface, gaseous exchange of soft tissues during healing process, barrier to prevent bacterial infections and it is completely non-Adherent. The use of BIOPAD® is painless for the patient and does not require removal or change of dressing. In this work, we evaluated the effect of BIOPAD® on fibroblasts behavior in term of cell viability, survival and growth and collagen production. The expression levels of some adhesion and traction-resistance related genes (ELN, DSP, FN1, FBN1, ITGB1, ITGA1, ITGA5, ITGA2, COL1A1, COL3A1) were analyzed using real time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR). All genes, except for ELN, DSP, ITGB1 and ITGA1 are up-regulated after 48 h of treatment. Altogether, our results point out the good potential of BIOPAD® as a biocompatible and regenerative tool in medicine.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalJournal of Biological Regulators and Homeostatic Agents
Volume31
Issue number2, Supplement 1
Publication statusPublished - Jun 1 2017

Fingerprint

Fibroblasts
Bandages
Cell Survival
Collagen
Granulation Tissue
Traction
Porifera
Growth
Humidity
Bacterial Infections
Tendons
Wound Healing
Genes
Horses
Reverse Transcription
Real-Time Polymerase Chain Reaction
Regeneration
Medicine
Polymerase Chain Reaction
Skin

Keywords

  • Bone
  • Expression
  • Gene
  • Mucosa
  • Regeneration

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy
  • Physiology
  • Immunology
  • Oncology
  • Endocrinology
  • Physiology (medical)
  • Cancer Research

Cite this

Genetic effects of biopad® on fibroblast primary culture. / Baj, A.; Beltramini, G. A.; Romano, M.; Lauritano, D.; Gaudio, R. M.; Palmieri, A.; Cura, F.; Gianni, A. B.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 31, No. 2, Supplement 1, 01.06.2017, p. 209-214.

Research output: Contribution to journalArticle

Baj, A. ; Beltramini, G. A. ; Romano, M. ; Lauritano, D. ; Gaudio, R. M. ; Palmieri, A. ; Cura, F. ; Gianni, A. B. / Genetic effects of biopad® on fibroblast primary culture. In: Journal of Biological Regulators and Homeostatic Agents. 2017 ; Vol. 31, No. 2, Supplement 1. pp. 209-214.
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