Abstract
Genetic immunization represents a new tool for investigating physiological and pathological immune responses. Here we used genetic immunization with naked DNA to study the immune relevance of aminoacid sequence homologies by evaluating the outcome of immunization to a viral protein homologous to an HLA molecule. The viral protein was Balf2, a protein of Epstein-Barr virus (EBV) that shares aminoacid sequence homology with the HLA allele DRB1*0801. After genetic immunization of BALB/c mice with a construct encoding Balf2, we analyzed T cell responses of immunized mice. We found that cross-reactive proliferative and cytotoxic responses were raised to the homologous sequence as expressed by HLA-DRB1*0801. Furthermore, preferential secretion of Th1-type pro-inflammatory cytokines occurred. This strategy can allow rapid screening of interactive immune networks involving aminoacid sequence homologies between organisms.
| Original language | English |
|---|---|
| Pages (from-to) | 105-110 |
| Number of pages | 6 |
| Journal | Autoimmunity |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Epstein-Barr virus (EBV)
- HLA
- Immunization
- Naked DNA
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Genetic immunization maps T cell (auto)immune responses to self antigens homologous to exogenous proteins. / La Cava, Antonio; Massa, Margherita; Mendivil, Alberto; Martini, Alberto; Albani, Salvatore.
In: Autoimmunity, Vol. 35, No. 2, 2002, p. 105-110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic immunization maps T cell (auto)immune responses to self antigens homologous to exogenous proteins
AU - La Cava, Antonio
AU - Massa, Margherita
AU - Mendivil, Alberto
AU - Martini, Alberto
AU - Albani, Salvatore
PY - 2002
Y1 - 2002
N2 - Genetic immunization represents a new tool for investigating physiological and pathological immune responses. Here we used genetic immunization with naked DNA to study the immune relevance of aminoacid sequence homologies by evaluating the outcome of immunization to a viral protein homologous to an HLA molecule. The viral protein was Balf2, a protein of Epstein-Barr virus (EBV) that shares aminoacid sequence homology with the HLA allele DRB1*0801. After genetic immunization of BALB/c mice with a construct encoding Balf2, we analyzed T cell responses of immunized mice. We found that cross-reactive proliferative and cytotoxic responses were raised to the homologous sequence as expressed by HLA-DRB1*0801. Furthermore, preferential secretion of Th1-type pro-inflammatory cytokines occurred. This strategy can allow rapid screening of interactive immune networks involving aminoacid sequence homologies between organisms.
AB - Genetic immunization represents a new tool for investigating physiological and pathological immune responses. Here we used genetic immunization with naked DNA to study the immune relevance of aminoacid sequence homologies by evaluating the outcome of immunization to a viral protein homologous to an HLA molecule. The viral protein was Balf2, a protein of Epstein-Barr virus (EBV) that shares aminoacid sequence homology with the HLA allele DRB1*0801. After genetic immunization of BALB/c mice with a construct encoding Balf2, we analyzed T cell responses of immunized mice. We found that cross-reactive proliferative and cytotoxic responses were raised to the homologous sequence as expressed by HLA-DRB1*0801. Furthermore, preferential secretion of Th1-type pro-inflammatory cytokines occurred. This strategy can allow rapid screening of interactive immune networks involving aminoacid sequence homologies between organisms.
KW - Epstein-Barr virus (EBV)
KW - HLA
KW - Immunization
KW - Naked DNA
UR - http://www.scopus.com/inward/record.url?scp=0036085664&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036085664&partnerID=8YFLogxK
U2 - 10.1080/08916930290016592
DO - 10.1080/08916930290016592
M3 - Article
C2 - 12071433
AN - SCOPUS:0036085664
VL - 35
SP - 105
EP - 110
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 2
ER -