Genetic polymorphism of N-acetyltransferases, glutathione S-transferase M1 and NAD(P)H: quinone oxidoreductase in relation to malignant and benign pancreatic disease risk

H. Bartsch, C. Malaveille, A. B. Lowenfels, P. Maisonneuve, A. Hautefeuille, P. Boyle, P. Banks, G. Cavallini, L. Domellöf, L. Gullo, E. Krag, P. G. Lankisch, C. S. Pitchumoni, A. L. Warshaw

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Abstract

Carcinogens present in cigarette smoke and diet have been associated with pancreatic cancer. We hypothesized that heterocyclic and aromatic amines implicated in these exposures could be involved as causative agents and that therefore genetic variation in enzymes metabolizing these carcinogens could modify the risk of developing malignant and benign pancreatic disease. The effect of the genetic polymorphism of acetyl-transferases (NAT1 and NAT2), glutathione S-transferase M1 (GSTM1) and NAD(P)H: quinone oxidoreductase 1 (NQO1) on the risk of pancreatic diseases (cancer, pancreatitis) was examined in a case-control study. PCR-based assays were used for genotype analysis of genomic DNA from whole blood cells. Samples collected from Caucasian patients with diagnosed pancreatic cancer (n = 81), with non-alcoholic (n = 41) and alcoholic pancreatitis (n = 73) and from asymptomatic control subjects (n = 78) were analysed. The prevalence of GSTM1 null genotype and of NAT2 fast and slow acetylator genotypes and the distribution of frequencies for NQO1 genotypes did not differ in subjects with pancreatic diseases vs controls. For NAT1 slow acetylators a non-significant excess (P = 0.18) was found among pancreatic cancer cases vs controls. There was a significant over- representation of the GSTM1 AB or B genotype in all pancreatic disease cases combined (OR = 2.6; P <0.05). When concurrent controls were pooled with literature controls (n = 1427), OR was 1.4 (P = 0.08). The results of this study, requiring confirmation, suggest that the polymorphism of GSTM1 and NAT1 enzymes may be associated with a modest increase in susceptibility to pancreatic disease.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalEuropean Journal of Cancer Prevention
Volume7
Issue number3
DOIs
Publication statusPublished - 1998

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Keywords

  • Alcoholic pancreatitis
  • Asymptomatic Caucasian subjects
  • Diet
  • Genetic polymorphism
  • Glutathione
  • heterocyclic/aromatic amines
  • N-acetyl-transferase
  • NAD(P)H:quinone oxidoreductase
  • Pancreatic cancer
  • S-transferase M1

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bartsch, H., Malaveille, C., Lowenfels, A. B., Maisonneuve, P., Hautefeuille, A., Boyle, P., Banks, P., Cavallini, G., Domellöf, L., Gullo, L., Krag, E., Lankisch, P. G., Pitchumoni, C. S., & Warshaw, A. L. (1998). Genetic polymorphism of N-acetyltransferases, glutathione S-transferase M1 and NAD(P)H: quinone oxidoreductase in relation to malignant and benign pancreatic disease risk. European Journal of Cancer Prevention, 7(3), 215-223. https://doi.org/10.1097/00008469-199806000-00006