Genetic variability of hepatitis C virus in HBV/HCV co-infection and HCV single-infection

M. S. De Mitri, G. Morsica, R. Cassini, S. Bagaglio, P. Andreone, G. Bianchi, M. Margotti, M. Bernardi

Research output: Contribution to journalArticle

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Abstract

To describe the virological profile of HCV in HBV/HCV co-infection, we investigated the variability of HVR-1 and NS5A domains, which may be involved in viral persistence and replication efficiency. We studied 95 patients: 37 with serological markers of HBV/HCV co-infection, 33 with single HBV and 25 with single HCV infection. HVR-1 complexity and NS5A gene variability were respectively explored by means of PCR-SSCP and direct sequencing. Serum HBV genomes were detected in all coinfected patients: 19 also had circulating HCV particles (group BC-I), whereas HCV were undetectable in the other 18 (group BC-II). Group BC-I was characterised by a significantly lower HBV replication capacity, that reflects the replicative dominance of HCV, although the dominant virus had the same degree of variability as the HCV in single infection. HBV viral load was higher in group BC-II, but not significantly different from that observed in the single infection. Our data indicate an alternation in replicative dominance in co-infection: HBV can suppress HCV replication to undetectable levels, whereas HCV may reduce but does not abrogate the replication capacity of HBV Furthermore, in the cases of HCV dominance, circulating HBV genomes did not have a significant effect on the viral heterogeneity of HCV.

Original languageEnglish
Pages (from-to)261-271
Number of pages11
JournalArchives of Virology
Volume150
Issue number2
DOIs
Publication statusPublished - Feb 2005

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Coinfection
Hepacivirus
Infection
Genome
Single-Stranded Conformational Polymorphism
Viral Load
Viruses
Polymerase Chain Reaction
Serum
Genes

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Genetic variability of hepatitis C virus in HBV/HCV co-infection and HCV single-infection. / De Mitri, M. S.; Morsica, G.; Cassini, R.; Bagaglio, S.; Andreone, P.; Bianchi, G.; Margotti, M.; Bernardi, M.

In: Archives of Virology, Vol. 150, No. 2, 02.2005, p. 261-271.

Research output: Contribution to journalArticle

De Mitri, MS, Morsica, G, Cassini, R, Bagaglio, S, Andreone, P, Bianchi, G, Margotti, M & Bernardi, M 2005, 'Genetic variability of hepatitis C virus in HBV/HCV co-infection and HCV single-infection', Archives of Virology, vol. 150, no. 2, pp. 261-271. https://doi.org/10.1007/s00705-004-0415-7
De Mitri, M. S. ; Morsica, G. ; Cassini, R. ; Bagaglio, S. ; Andreone, P. ; Bianchi, G. ; Margotti, M. ; Bernardi, M. / Genetic variability of hepatitis C virus in HBV/HCV co-infection and HCV single-infection. In: Archives of Virology. 2005 ; Vol. 150, No. 2. pp. 261-271.
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