Genetic variation at 16q24.2 is associated with small vessel stroke

Matthew Traylor, Rainer Malik, Mike A. Nalls, Ioana Cotlarciuc, Farid Radmanesh, Farid Radmanesh, Farid Radmanesh, Gudmar Thorleifsson, Ken B. Hanscombe, Carl Langefeld, Danish Saleheen, Natalia S. Rost, Idil Yet, Tim D. Spector, Jordana T. Bell, Eilis Hannon, Jonathan Mill, Jonathan Mill, Ganesh Chauhan, Ganesh ChauhanStephanie Debette, Stephanie Debette, Joshua C. Bis, W. T. Longstreth, M. Arfan Ikram, Lenore J. Launer, Sudha Seshadri, Sudha Seshadri, Monica Anne Hamilton-Bruce, Jordi Jimenez-Conde, John W. Cole, Reinhold Schmidt, Agnieszka Słowik, Robin Lemmens, Robin Lemmens, Robin Lemmens, Arne Lindgren, Arne Lindgren, Olle Melander, Raji P. Grewal, Ralph L. Sacco, Tatjana Rundek, Kathryn Rexrode, Donna K. Arnett, Julie A. Johnson, Julie A. Johnson, Oscar R. Benavente, Sylvia Wasssertheil-Smoller, Jin Moo Lee, Sara L. Pulit, Quenna Wong, Stephen S. Rich, Paul I.W. de Bakker, Paul I.W. de Bakker, Patrick F. McArdle, Daniel Woo, Christopher D. Anderson, Christopher D. Anderson, Christopher D. Anderson, Christopher D. Anderson, Huichun Xu, Laura Heitsch, Myriam Fornage, Christina Jern, Kari Stefansson, Kari Stefansson, Unnur Thorsteinsdottir, Unnur Thorsteinsdottir, Solveig Gretarsdottir, Cathryn M. Lewis, Cathryn M. Lewis, Pankaj Sharma, Cathie L.M. Sudlow, Peter M. Rothwell, Giorgio B. Boncoraglio, Vincent Thijs, Vincent Thijs, Vincent Thijs, Chris Levi, James F. Meschia, Jonathan Rosand, Jonathan Rosand, Jonathan Rosand, Jonathan Rosand, Steven J. Kittner, Braxton D. Mitchell, Braxton D. Mitchell, Martin Dichgans, Martin Dichgans, Bradford B. Worrall, Hugh S. Markus

Research output: Contribution to journalArticlepeer-review


© 2016 The Authors. Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association. Objective: Genome-wide association studies (GWAS) have been successful at identifying associations with stroke and stroke subtypes, but have not yet identified any associations solely with small vessel stroke (SVS). SVS comprises one quarter of all ischemic stroke and is a major manifestation of cerebral small vessel disease, the primary cause of vascular cognitive impairment. Studies across neurological traits have shown that younger-onset cases have an increased genetic burden. We leveraged this increased genetic burden by performing an age-at-onset informed GWAS meta-analysis, including a large younger-onset SVS population, to identify novel associations with stroke. Methods: We used a three-stage age-at-onset informed GWAS to identify novel genetic variants associated with stroke. On identifying a novel locus associated with SVS, we assessed its influence on other small vessel disease phenotypes, as well as on messenger RNA (mRNA) expression of nearby genes, and on DNA methylation of nearby CpG sites in whole blood and in the fetal brain. Results: We identified an association with SVS in 4,203 cases and 50,728 controls on chromosome 16q24.2 (odds ratio [OR; 95% confidence interval {CI}] = 1.16 [1.10–1.22] ; p = 3.2 × 10 −9 ). The lead single-nucleotide polymorphism (rs12445022) was also associated with cerebral white matter hyperintensities (OR [95% CI] = 1.10 [1.05–1.16] ; p = 5.3 × 10 −5 ; N = 3,670), but not intracerebral hemorrhage (OR [95% CI] = 0.97 [0.84–1.12] ; p = 0.71; 1,545 cases, 1,481 controls). rs12445022 is associated with mRNA expression of ZCCHC14 in arterial tissues (p = 9.4 × 10 −7 ) and DNA methylation at probe cg16596957 in whole blood (p = 5.3 × 10 −6 ). Interpretation: 16q24.2 is associated with SVS. Associations of the locus with expression of ZCCHC14 and DNA methylation suggest the locus acts through changes to regulatory elements. Ann Neurol 2017;81:383–394.
Original languageEnglish
Pages (from-to)383-394
Number of pages12
JournalAnnals of Neurology
Issue number3
Publication statusPublished - Mar 1 2017


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