Genistein in the control of breast cancer cell growth: Insights into the mechanism of action in vitro

Laura Fioravanti, Vera Cappelletti, Patrizia Miodini, Enrico Ronchi, Moreno Brivio, Giovanni Di Fronzo

Research output: Contribution to journalArticle

Abstract

Genistein significantly inhibited cell growth (IC50 around 10 μM) of MCF-7, MDAMB-231 and HBL-100 cell lines, but not of skin-derived fibroblasts and counteracted the growth-stimulatory effects exerted by estradiol and growth factors. It abolished the paracrine stimulation observed in MCF-7 cells in co-culture with MDAMB-231 or fibroblasts. Genistein-treated cells accumulated in the S and G2/M phases of the cell cycle and underwent apoptosis. Genistein decreased tyrosine phosphorylation induced upon treatment with transforming growth factor-α. Finally, genistein bound the estrogen receptor (ER) (relative affinity constant K(d) = 4 nM), induced pS2 and cathepsin-D transcription and increased nuclear ER levels.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalCancer Letters
Volume130
Issue number1-2
DOIs
Publication statusPublished - Aug 14 1998

Keywords

  • Breast cancer
  • Cell cycle
  • Estradiol
  • Genistein
  • Growth factors
  • Tyrosine kinase inhibitors

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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