Genome distribution, chromosomal allocation, and organization of the major and minor satellite dnas in 11 species and subspecies of the genus mus

S. Garagna, C. A. Redi, E. Capanna, N. Andayani, R. M. Alfano, P. Doi, G. Viale

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We compared the genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs (satDNAs) in 11 species and subspecies of the genus Mus. Southern blot analysis of the major and minor satDNAs showed similar fragment profiles in all 11 species, with the exception of M. cervicolor and M. cookii for the major satDNAs and M. caroli, M. cervicolor, and M. cookii for the minor satDNAs, where these sequences could not be detected by the probes used. In situ hybridization of the major and minor satDNA probes revealed chromosome-specific allocations of these sequences with quantitative species-specific patterns. Fluorometric analysis of the organization of the satellite sequences suggested that in the M. domesticus genome satDNA sequences are clustered in tandem repeats that are longer than those present in other Mus genomes. When compared with the other Mus genomes so far studied, the domesticus genome shows the highest quantity of satDNA sequences with a long-range organization of satDNA sequences.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalCytogenetic and Genome Research
Volume64
Issue number3-4
DOIs
Publication statusPublished - 1993

Fingerprint

Satellite DNA
Genome
Fluorometry
Tandem Repeat Sequences
Southern Blotting
In Situ Hybridization
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Cell Biology

Cite this

Genome distribution, chromosomal allocation, and organization of the major and minor satellite dnas in 11 species and subspecies of the genus mus. / Garagna, S.; Redi, C. A.; Capanna, E.; Andayani, N.; Alfano, R. M.; Doi, P.; Viale, G.

In: Cytogenetic and Genome Research, Vol. 64, No. 3-4, 1993, p. 247-255.

Research output: Contribution to journalArticle

@article{674220fdfe1444a99ed34120bc18d2e5,
title = "Genome distribution, chromosomal allocation, and organization of the major and minor satellite dnas in 11 species and subspecies of the genus mus",
abstract = "We compared the genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs (satDNAs) in 11 species and subspecies of the genus Mus. Southern blot analysis of the major and minor satDNAs showed similar fragment profiles in all 11 species, with the exception of M. cervicolor and M. cookii for the major satDNAs and M. caroli, M. cervicolor, and M. cookii for the minor satDNAs, where these sequences could not be detected by the probes used. In situ hybridization of the major and minor satDNA probes revealed chromosome-specific allocations of these sequences with quantitative species-specific patterns. Fluorometric analysis of the organization of the satellite sequences suggested that in the M. domesticus genome satDNA sequences are clustered in tandem repeats that are longer than those present in other Mus genomes. When compared with the other Mus genomes so far studied, the domesticus genome shows the highest quantity of satDNA sequences with a long-range organization of satDNA sequences.",
author = "S. Garagna and Redi, {C. A.} and E. Capanna and N. Andayani and Alfano, {R. M.} and P. Doi and G. Viale",
year = "1993",
doi = "10.1159/000133587",
language = "English",
volume = "64",
pages = "247--255",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "3-4",

}

TY - JOUR

T1 - Genome distribution, chromosomal allocation, and organization of the major and minor satellite dnas in 11 species and subspecies of the genus mus

AU - Garagna, S.

AU - Redi, C. A.

AU - Capanna, E.

AU - Andayani, N.

AU - Alfano, R. M.

AU - Doi, P.

AU - Viale, G.

PY - 1993

Y1 - 1993

N2 - We compared the genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs (satDNAs) in 11 species and subspecies of the genus Mus. Southern blot analysis of the major and minor satDNAs showed similar fragment profiles in all 11 species, with the exception of M. cervicolor and M. cookii for the major satDNAs and M. caroli, M. cervicolor, and M. cookii for the minor satDNAs, where these sequences could not be detected by the probes used. In situ hybridization of the major and minor satDNA probes revealed chromosome-specific allocations of these sequences with quantitative species-specific patterns. Fluorometric analysis of the organization of the satellite sequences suggested that in the M. domesticus genome satDNA sequences are clustered in tandem repeats that are longer than those present in other Mus genomes. When compared with the other Mus genomes so far studied, the domesticus genome shows the highest quantity of satDNA sequences with a long-range organization of satDNA sequences.

AB - We compared the genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs (satDNAs) in 11 species and subspecies of the genus Mus. Southern blot analysis of the major and minor satDNAs showed similar fragment profiles in all 11 species, with the exception of M. cervicolor and M. cookii for the major satDNAs and M. caroli, M. cervicolor, and M. cookii for the minor satDNAs, where these sequences could not be detected by the probes used. In situ hybridization of the major and minor satDNA probes revealed chromosome-specific allocations of these sequences with quantitative species-specific patterns. Fluorometric analysis of the organization of the satellite sequences suggested that in the M. domesticus genome satDNA sequences are clustered in tandem repeats that are longer than those present in other Mus genomes. When compared with the other Mus genomes so far studied, the domesticus genome shows the highest quantity of satDNA sequences with a long-range organization of satDNA sequences.

UR - http://www.scopus.com/inward/record.url?scp=0027451779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027451779&partnerID=8YFLogxK

U2 - 10.1159/000133587

DO - 10.1159/000133587

M3 - Article

VL - 64

SP - 247

EP - 255

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 3-4

ER -