Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition

Jing Chen, Collin Melton, Nayoung Suh, Jeong Su Oh, Kathleen Horner, Fang Xie, Claudio Sette, Robert Blelloch, Marco Conti

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Oocyte maturation, fertilization, and early embryonic development occur in the absence of gene transcription. Therefore, it is critical to understand at a global level the post-transcriptional events that are driving these transitions. Here we used a systems approach by combining polysome mRNA profiling and bioinformatics to identify RNA-binding motifs in mRNAs that either enter or exit the polysome pool during mouse oocyte maturation. Association of mRNA with the polysomes correlates with active translation. Using this strategy, we identified highly specific patterns of mRNA recruitment to the polysomes that are synchronized with the cell cycle. A large number of the mRNAs recovered with translating ribosomes contain motifs for the RNA-binding proteins DAZL (deleted in azoospermia-like) and CPEB (cytoplasmic polyadenylation element-binding protein). Although a Dazl role in early germ cell development is well established, no function has been described during oocyte-to-embryo transition. We demonstrate that CPEB1 regulates Dazl post-transcriptionally, and that DAZL is essential for meiotic maturation and embryonic cleavage. In the absence of DAZL synthesis, the meiotic spindle fails to form due to disorganization of meiotic microtubules. Therefore, Cpeb1 and Dazl function in a progressive, self-reinforcing pathway to promote oocyte maturation and early embryonic development.

Original languageEnglish
Pages (from-to)755-766
Number of pages12
JournalGenes and Development
Volume25
Issue number7
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Azoospermia
Zygote
Oocytes
Polyribosomes
Genome
Messenger RNA
Embryonic Development
Polyadenylation
Spindle Apparatus
RNA-Binding Proteins
Systems Analysis
Computational Biology
Ribosomes
Fertilization
Germ Cells
Microtubules
Cell Cycle
Carrier Proteins
Embryonic Structures
Genes

Keywords

  • Maternal mRNAs
  • Meiosis
  • Oocyte
  • Translation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition. / Chen, Jing; Melton, Collin; Suh, Nayoung; Oh, Jeong Su; Horner, Kathleen; Xie, Fang; Sette, Claudio; Blelloch, Robert; Conti, Marco.

In: Genes and Development, Vol. 25, No. 7, 04.2011, p. 755-766.

Research output: Contribution to journalArticle

Chen, Jing ; Melton, Collin ; Suh, Nayoung ; Oh, Jeong Su ; Horner, Kathleen ; Xie, Fang ; Sette, Claudio ; Blelloch, Robert ; Conti, Marco. / Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition. In: Genes and Development. 2011 ; Vol. 25, No. 7. pp. 755-766.
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