Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population

Yafang Li, Xiangjun Xiao, Younghun Han, Olga Gorlova, David Qian, Natasha Leighl, Jakob S. Johansen, Matt Barnett, Chu Chen, Gary Goodman, Angela Cox, Fiona Taylor, Penella Woll, H. Erich Wichmann, Judith Manz, Thomas Muley, Angela Risch, Albert Rosenberger, Susanne M. Arnold, Eric B. HauraCiprian Bolca, Ivana Holcatova, Vladimir Janout, Milica Kontic, Jolanta Lissowska, Anush Mukeria, Simona Ognjanovic, Tadeusz M. Orlowski, Ghislaine Scelo, Beata Swiatkowska, David Zaridze, Per Bakke, Vidar Skaug, Shanbeh Zienolddiny, Eric J. Duell, Lesley M. Butler, Richard Houlston, María Soler Artigas, Kjell Grankvist, Mikael Johansson, Frances A. Shepherd, Michael W. Marcus, Hans Brunnström, Jonas Manjer, Olle Melander, David C. Muller, Kim Overvad, Antonia Trichopoulou, Pier Alberto Bertazzi, Angela C. Pesatori, David C. Christiani, Neil Caporaso

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Non-small cell lung cancer is the most common type of lung cancer. Both environmental and genetic risk factors contribute to lung carcinogenesis. We conducted a genome-wide interaction analysis between single nucleotide polymorphisms (SNPs) and smoking status (never- versus ever-smokers) in a European-descent population. We adopted a two-step analysis strategy in the discovery stage: we first conducted a case-only interaction analysis to assess the relationship between SNPs and smoking behavior using 13 336 non-small cell lung cancer cases. Candidate SNPs with P-value <0.001 were further analyzed using a standard case-control interaction analysis including 13 970 controls. The significant SNPs with P-value <3.5 × 10-5 (correcting for multiple tests) from the case-control analysis in the discovery stage were further validated using an independent replication dataset comprising 5377 controls and 3054 non-small cell lung cancer cases. We further stratified the analysis by histological subtypes. Two novel SNPs, rs6441286 and rs17723637, were identified for overall lung cancer risk. The interaction odds ratio and meta-analysis P-value for these two SNPs were 1.24 with 6.96 × 10-7 and 1.37 with 3.49 × 10-7, respectively. In addition, interaction of smoking with rs4751674 was identified in squamous cell lung carcinoma with an odds ratio of 0.58 and P-value of 8.12 × 10-7. This study is by far the largest genome-wide SNP-smoking interaction analysis reported for lung cancer. The three identified novel SNPs provide potential candidate biomarkers for lung cancer risk screening and intervention. The results from our study reinforce that gene-smoking interactions play important roles in the etiology of lung cancer and account for part of the missing heritability of this disease.

Original languageEnglish
Pages (from-to)336-346
Number of pages11
JournalCarcinogenesis
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 8 2018

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Non-Small Cell Lung Carcinoma
Single Nucleotide Polymorphism
Smoking
Genome
Lung Neoplasms
Population
Odds Ratio
Lung
Early Detection of Cancer
Meta-Analysis
Squamous Cell Carcinoma
Carcinogenesis
Biomarkers
Genes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Li, Y., Xiao, X., Han, Y., Gorlova, O., Qian, D., Leighl, N., ... Caporaso, N. (2018). Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population. Carcinogenesis, 39(3), 336-346. https://doi.org/10.1093/carcin/bgx113

Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population. / Li, Yafang; Xiao, Xiangjun; Han, Younghun; Gorlova, Olga; Qian, David; Leighl, Natasha; Johansen, Jakob S.; Barnett, Matt; Chen, Chu; Goodman, Gary; Cox, Angela; Taylor, Fiona; Woll, Penella; Wichmann, H. Erich; Manz, Judith; Muley, Thomas; Risch, Angela; Rosenberger, Albert; Arnold, Susanne M.; Haura, Eric B.; Bolca, Ciprian; Holcatova, Ivana; Janout, Vladimir; Kontic, Milica; Lissowska, Jolanta; Mukeria, Anush; Ognjanovic, Simona; Orlowski, Tadeusz M.; Scelo, Ghislaine; Swiatkowska, Beata; Zaridze, David; Bakke, Per; Skaug, Vidar; Zienolddiny, Shanbeh; Duell, Eric J.; Butler, Lesley M.; Houlston, Richard; Artigas, María Soler; Grankvist, Kjell; Johansson, Mikael; Shepherd, Frances A.; Marcus, Michael W.; Brunnström, Hans; Manjer, Jonas; Melander, Olle; Muller, David C.; Overvad, Kim; Trichopoulou, Antonia; Bertazzi, Pier Alberto; Pesatori, Angela C.; Christiani, David C.; Caporaso, Neil.

In: Carcinogenesis, Vol. 39, No. 3, 08.03.2018, p. 336-346.

Research output: Contribution to journalArticle

Li, Y, Xiao, X, Han, Y, Gorlova, O, Qian, D, Leighl, N, Johansen, JS, Barnett, M, Chen, C, Goodman, G, Cox, A, Taylor, F, Woll, P, Wichmann, HE, Manz, J, Muley, T, Risch, A, Rosenberger, A, Arnold, SM, Haura, EB, Bolca, C, Holcatova, I, Janout, V, Kontic, M, Lissowska, J, Mukeria, A, Ognjanovic, S, Orlowski, TM, Scelo, G, Swiatkowska, B, Zaridze, D, Bakke, P, Skaug, V, Zienolddiny, S, Duell, EJ, Butler, LM, Houlston, R, Artigas, MS, Grankvist, K, Johansson, M, Shepherd, FA, Marcus, MW, Brunnström, H, Manjer, J, Melander, O, Muller, DC, Overvad, K, Trichopoulou, A, Bertazzi, PA, Pesatori, AC, Christiani, DC & Caporaso, N 2018, 'Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population', Carcinogenesis, vol. 39, no. 3, pp. 336-346. https://doi.org/10.1093/carcin/bgx113
Li, Yafang ; Xiao, Xiangjun ; Han, Younghun ; Gorlova, Olga ; Qian, David ; Leighl, Natasha ; Johansen, Jakob S. ; Barnett, Matt ; Chen, Chu ; Goodman, Gary ; Cox, Angela ; Taylor, Fiona ; Woll, Penella ; Wichmann, H. Erich ; Manz, Judith ; Muley, Thomas ; Risch, Angela ; Rosenberger, Albert ; Arnold, Susanne M. ; Haura, Eric B. ; Bolca, Ciprian ; Holcatova, Ivana ; Janout, Vladimir ; Kontic, Milica ; Lissowska, Jolanta ; Mukeria, Anush ; Ognjanovic, Simona ; Orlowski, Tadeusz M. ; Scelo, Ghislaine ; Swiatkowska, Beata ; Zaridze, David ; Bakke, Per ; Skaug, Vidar ; Zienolddiny, Shanbeh ; Duell, Eric J. ; Butler, Lesley M. ; Houlston, Richard ; Artigas, María Soler ; Grankvist, Kjell ; Johansson, Mikael ; Shepherd, Frances A. ; Marcus, Michael W. ; Brunnström, Hans ; Manjer, Jonas ; Melander, Olle ; Muller, David C. ; Overvad, Kim ; Trichopoulou, Antonia ; Bertazzi, Pier Alberto ; Pesatori, Angela C. ; Christiani, David C. ; Caporaso, Neil. / Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population. In: Carcinogenesis. 2018 ; Vol. 39, No. 3. pp. 336-346.
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AU - Qian, David

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AU - Orlowski, Tadeusz M.

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AU - Swiatkowska, Beata

AU - Zaridze, David

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N2 - Non-small cell lung cancer is the most common type of lung cancer. Both environmental and genetic risk factors contribute to lung carcinogenesis. We conducted a genome-wide interaction analysis between single nucleotide polymorphisms (SNPs) and smoking status (never- versus ever-smokers) in a European-descent population. We adopted a two-step analysis strategy in the discovery stage: we first conducted a case-only interaction analysis to assess the relationship between SNPs and smoking behavior using 13 336 non-small cell lung cancer cases. Candidate SNPs with P-value <0.001 were further analyzed using a standard case-control interaction analysis including 13 970 controls. The significant SNPs with P-value <3.5 × 10-5 (correcting for multiple tests) from the case-control analysis in the discovery stage were further validated using an independent replication dataset comprising 5377 controls and 3054 non-small cell lung cancer cases. We further stratified the analysis by histological subtypes. Two novel SNPs, rs6441286 and rs17723637, were identified for overall lung cancer risk. The interaction odds ratio and meta-analysis P-value for these two SNPs were 1.24 with 6.96 × 10-7 and 1.37 with 3.49 × 10-7, respectively. In addition, interaction of smoking with rs4751674 was identified in squamous cell lung carcinoma with an odds ratio of 0.58 and P-value of 8.12 × 10-7. This study is by far the largest genome-wide SNP-smoking interaction analysis reported for lung cancer. The three identified novel SNPs provide potential candidate biomarkers for lung cancer risk screening and intervention. The results from our study reinforce that gene-smoking interactions play important roles in the etiology of lung cancer and account for part of the missing heritability of this disease.

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