Genome-wide profiling identifies the THYT1 signature as a distinctive feature of widely metastatic Papillary Thyroid Carcinomas

Greta Gandolfi, Moira Ragazzi, Dario de Biase, Michela Visani, Eleonora Zanetti, Federica Torricelli, Valentina Sancisi, Mila Gugnoni, Gloria Manzotti, Luca Braglia, Silvio Cavuto, Domenico Franco Merlo, Giovanni Tallini, Andrea Frasoldati, Simonetta Piana, Alessia Ciarrocchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Papillary Thyroid Carcinomas (PTCs) are generally indolent tumors. However, a small but significant percentage of PTCs behaves aggressively, progressing to a diffuse metastatic spreading and leading to patient's death. The lack of reliable markers for predicting the metastatic behavior of these tumors prevents a correct risk based stratification of the disease, thus contributing to the issue of patients' overtreatment. In this study we aimed at identifying genetic features associated with the development of distant metastasis in PTCs. Results: We showed that DM PTCs are characterized by a moderate degree of copy number alterations but display low level of microsatellite instability and a low mutational burden. We identified duplication of Chr1q, duplication of Chr5p harboring the TERT genomic locus and mutations of TERT promoter as distinctive features of DM PTCs. These three genetic variables defined a signature (THYT1) that was significantly associated with a metastatic behavior and a shortened survival. We analyzed the THYT1 signature in PTCs fine needle aspirate biopsies (FNAB) and we demonstrating the applicability of this signature as a molecular marker in the pre-operative diagnostic setting of PTCs. Materials and Methods: A consecutive series of 2,937 thyroid malignancies, diagnosed at the Arcispedale S. Maria Nuova - IRCCS, Italy between 1978 and 2015 were searched to retrieve those who developed distant metastasis (DM, n = 50). We performed a deep profiling to explore the genomic landscape of these tumors. Conclusions: Overall our data identify the first genetic signature that independently predicts metastasis and negative outcome of PTCs, and lay the basis for the possible application of the THYT1 as prognostic marker to improve risk-based stratification and management of PTC patients.

Original languageEnglish
Pages (from-to)1813-1825
Number of pages13
JournalOncotarget
Volume9
Issue number2
DOIs
Publication statusPublished - Jan 1 2018

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Genome
Neoplasm Metastasis
Neoplasms
Papillary Thyroid cancer
Microsatellite Instability
Fine Needle Biopsy
Italy
Thyroid Gland
Mutation
Survival

Keywords

  • Copy number alterations
  • Distant metastases
  • Next generation sequencing
  • Papillary thyroid carcinomas
  • TERT duplication

ASJC Scopus subject areas

  • Oncology

Cite this

Genome-wide profiling identifies the THYT1 signature as a distinctive feature of widely metastatic Papillary Thyroid Carcinomas. / Gandolfi, Greta; Ragazzi, Moira; de Biase, Dario; Visani, Michela; Zanetti, Eleonora; Torricelli, Federica; Sancisi, Valentina; Gugnoni, Mila; Manzotti, Gloria; Braglia, Luca; Cavuto, Silvio; Merlo, Domenico Franco; Tallini, Giovanni; Frasoldati, Andrea; Piana, Simonetta; Ciarrocchi, Alessia.

In: Oncotarget, Vol. 9, No. 2, 01.01.2018, p. 1813-1825.

Research output: Contribution to journalArticle

Gandolfi, G, Ragazzi, M, de Biase, D, Visani, M, Zanetti, E, Torricelli, F, Sancisi, V, Gugnoni, M, Manzotti, G, Braglia, L, Cavuto, S, Merlo, DF, Tallini, G, Frasoldati, A, Piana, S & Ciarrocchi, A 2018, 'Genome-wide profiling identifies the THYT1 signature as a distinctive feature of widely metastatic Papillary Thyroid Carcinomas', Oncotarget, vol. 9, no. 2, pp. 1813-1825. https://doi.org/10.18632/oncotarget.22805
Gandolfi, Greta ; Ragazzi, Moira ; de Biase, Dario ; Visani, Michela ; Zanetti, Eleonora ; Torricelli, Federica ; Sancisi, Valentina ; Gugnoni, Mila ; Manzotti, Gloria ; Braglia, Luca ; Cavuto, Silvio ; Merlo, Domenico Franco ; Tallini, Giovanni ; Frasoldati, Andrea ; Piana, Simonetta ; Ciarrocchi, Alessia. / Genome-wide profiling identifies the THYT1 signature as a distinctive feature of widely metastatic Papillary Thyroid Carcinomas. In: Oncotarget. 2018 ; Vol. 9, No. 2. pp. 1813-1825.
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AU - Visani, Michela

AU - Zanetti, Eleonora

AU - Torricelli, Federica

AU - Sancisi, Valentina

AU - Gugnoni, Mila

AU - Manzotti, Gloria

AU - Braglia, Luca

AU - Cavuto, Silvio

AU - Merlo, Domenico Franco

AU - Tallini, Giovanni

AU - Frasoldati, Andrea

AU - Piana, Simonetta

AU - Ciarrocchi, Alessia

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N2 - Background: Papillary Thyroid Carcinomas (PTCs) are generally indolent tumors. However, a small but significant percentage of PTCs behaves aggressively, progressing to a diffuse metastatic spreading and leading to patient's death. The lack of reliable markers for predicting the metastatic behavior of these tumors prevents a correct risk based stratification of the disease, thus contributing to the issue of patients' overtreatment. In this study we aimed at identifying genetic features associated with the development of distant metastasis in PTCs. Results: We showed that DM PTCs are characterized by a moderate degree of copy number alterations but display low level of microsatellite instability and a low mutational burden. We identified duplication of Chr1q, duplication of Chr5p harboring the TERT genomic locus and mutations of TERT promoter as distinctive features of DM PTCs. These three genetic variables defined a signature (THYT1) that was significantly associated with a metastatic behavior and a shortened survival. We analyzed the THYT1 signature in PTCs fine needle aspirate biopsies (FNAB) and we demonstrating the applicability of this signature as a molecular marker in the pre-operative diagnostic setting of PTCs. Materials and Methods: A consecutive series of 2,937 thyroid malignancies, diagnosed at the Arcispedale S. Maria Nuova - IRCCS, Italy between 1978 and 2015 were searched to retrieve those who developed distant metastasis (DM, n = 50). We performed a deep profiling to explore the genomic landscape of these tumors. Conclusions: Overall our data identify the first genetic signature that independently predicts metastasis and negative outcome of PTCs, and lay the basis for the possible application of the THYT1 as prognostic marker to improve risk-based stratification and management of PTC patients.

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