Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma

Marina Scintu, Rita Vitale, Maria Prencipe, Antonietta Pia Gallo, Loriana Bonghi, Vanna Maria Valori, Evaristo Maiello, Monica Rinaldi, Emanuela Signori, Carla Rabitti, Massimo Carella, Bruno Dallapiccola, Vittorio Altomare, Vito M. Fazio, Paola Parrella

Research output: Contribution to journalArticlepeer-review

Abstract

In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P <0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P <0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r = 0.722, P = 0.018).

Original languageEnglish
Pages (from-to)298-307
Number of pages10
JournalCancer Letters
Volume254
Issue number2
DOIs
Publication statusPublished - Sep 8 2007

Keywords

  • Breast cancer
  • BUB1B
  • Gene expression
  • Genomic instability
  • MAD2L1

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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