Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis

Linda Moskovszky, Károly Szuhai, Tibor Krenács, Pancras C W Hogendoorn, Miklós Szendroi, Maria Serena Benassi, László Kopper, Tibor Füle, Zoltán Sápi

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Abstract

Genetic instability in relation to clinical behavior was studied in 52 cases of giant cell tumor of bone (GCTB). Ploidy was determined in the mononuclear cell population by using native cell smears and image cytometry. A relocalization technique allowed fluorescent in situ hybridization (FISH) analysis of CD68-negative neoplastic cells for numerical changes of chromosomes X, 3, 4, 6, 11, and telomeric association on 11p. Genome-wide alterations were tested using array comparative genomic hybridization (array-CGH) on magnetically separated CD68-negative tumor cells. CTNNB1, TP53, and BCL2 protein expression was also analyzed in formol-paraffin sections to see if their pathways are involved in the development of chromosomal instability. CD68-positive histiocytes showed no significant numerical chromosome and telomeric alterations. Based on ploidy values and clinical outcome, we could distinguish five groups as follows: diploid nonrecurrent (n = 20), tetraploid nonrecurrent (n = 6), diploid recurrent (n = 5), tetraploid and/or aneuploid recurrent (n = 14), and malignant cases (n = 7). Random individual-cell aneusomy was significantly (P <0.001) more frequent in the recurrent groups (36.01 ± 11.94%) than in the benign nonrecurrent cases (10.65 ± 3.66%). The diploid recurrent group showed significantly (P <0.001) increased balanced aneusomy compared with the diploid nonrecurrent group and the tetraploid nonrecurrent group represented eusomic polysomy. Array-CGH and FISH showed clonal aberrations almost exclusively in the malignant group. None of the protein markers tested showed significant correlation with elevated aneuploidy/polysomy (P = 0.56). Our results show that ploidy determination combined with FISH analysis may help predicting recurrence potential of GCTB and suggest that chromosomal abnormalities superimposed on telomeric associations could be responsible for an aggressive clinical course.

Original languageEnglish
Pages (from-to)468-479
Number of pages12
JournalGenes Chromosomes and Cancer
Volume48
Issue number6
DOIs
Publication statusPublished - Jun 2009

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Giant Cell Tumor of Bone
Comparative Genomic Hybridization
Ploidies
Genomic Instability
Fluorescence In Situ Hybridization
Diploidy
Tetraploidy
DNA
Aneuploidy
Proto-Oncogene Proteins c-bcl-2
Image Cytometry
Tumor Suppressor Protein p53
Chromosomal Instability
Chromosomes, Human, Pair 3
Histiocytes
Chromosome Aberrations
Paraffin
Formaldehyde
Chromosomes
Genome

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Medicine(all)

Cite this

Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis. / Moskovszky, Linda; Szuhai, Károly; Krenács, Tibor; Hogendoorn, Pancras C W; Szendroi, Miklós; Benassi, Maria Serena; Kopper, László; Füle, Tibor; Sápi, Zoltán.

In: Genes Chromosomes and Cancer, Vol. 48, No. 6, 06.2009, p. 468-479.

Research output: Contribution to journalArticle

Moskovszky, L, Szuhai, K, Krenács, T, Hogendoorn, PCW, Szendroi, M, Benassi, MS, Kopper, L, Füle, T & Sápi, Z 2009, 'Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis', Genes Chromosomes and Cancer, vol. 48, no. 6, pp. 468-479. https://doi.org/10.1002/gcc.20656
Moskovszky, Linda ; Szuhai, Károly ; Krenács, Tibor ; Hogendoorn, Pancras C W ; Szendroi, Miklós ; Benassi, Maria Serena ; Kopper, László ; Füle, Tibor ; Sápi, Zoltán. / Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis. In: Genes Chromosomes and Cancer. 2009 ; Vol. 48, No. 6. pp. 468-479.
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AU - Szendroi, Miklós

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