Genomic organisation of the mouse ret proto-oncogene

Danilo Panetta, Luo Yin, Roberto Barale, Giovanni Romeo, Roberto Ravazzolo, Isabella Ceccherinp, Aldamaria Pulit

Research output: Contribution to journalArticlepeer-review

Abstract

The RET proto-oncogene is involved in the development of both kidney and neural crests derived tissues. RET deleterious mutations cause hereditary neuroendocrine tumours and congenital intestinal agangliono-sis. Ongoing efforts aimed at elucidating the function of this gene include expression studies in different species and in transgenic mice. As first step in the study of Ret expression in mouse, we obtained the mouse Ret genomic structure. Intron-exon boundaries were determined and sequenced, all introns but the first one were amplified and cloned, and exons positioned in a restriction map. Mouse and human genes comparison indicates a highly conserved genomic organisation, except for exon 21 which is not conserved in mouse. A region extending 386 bp 5′ to the first exon was sequenced and compared with its human counterpart. Some features, reported for the human promoter, like the absence of TATA or CAAT boxes and a high GC content, are conserved.

Original languageEnglish
Pages (from-to)501-506
Number of pages6
JournalMitochondrial DNA
Volume11
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • genomic structure
  • HSCR
  • human/mouse sequence comparison
  • MEN2
  • Ref proto-oncogene

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

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