Abstract
Caveolin-1 and caveolin-2 are related proteins involved in the biogenesis of caveolae. The corresponding genes in humans (CAV and CAV2, respectively), have been mapped to a common locus in chromosome 7q31.1, and are possible candidates for the tumor suppressor gene postulated in this region. Here, we show that CAV and CAV2 are independent transcriptional units lying in the same orientation, with CAV2 centromeric and about 17 kb upstream to CAV. The two genes have similar tissue expression patterns. Alternative termination/polyadenylation generates two CAV2 mRNAs. Multiple transcriptional start sites spanning 35 bp upstream from the CAV2 ATG are detected by 5' RACE, consistent with a TATA-less promoter predicted by sequence analysis. The CAV2 promoter region contains two SRE-like boxes resembling those described in the CAV promoter and proposed to link transcription to intracellular cholesterol levels. However, exogenous sterols had only minor effects on CAV and CAV2 RNA levels in HeLa cells, suggesting that SREBPs are not sufficient to regulate caveolin transcription. (C) 2000 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 75-83 |
| Number of pages | 9 |
| Journal | Gene |
| Volume | 243 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Feb 8 2000 |
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Keywords
- Caveolae
- Cholesterol homeostasis
- Sterol responsive element
- Tumor suppressor
ASJC Scopus subject areas
- Genetics
Cite this
Genomic organization and transcriptional analysis of the human genes coding for caveolin-1 and caveolin-2. / Fra, Anna M.; Pasqualetto, Elena; Mancini, Mita; Sitia, Roberto.
In: Gene, Vol. 243, No. 1-2, 08.02.2000, p. 75-83.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic organization and transcriptional analysis of the human genes coding for caveolin-1 and caveolin-2
AU - Fra, Anna M.
AU - Pasqualetto, Elena
AU - Mancini, Mita
AU - Sitia, Roberto
PY - 2000/2/8
Y1 - 2000/2/8
N2 - Caveolin-1 and caveolin-2 are related proteins involved in the biogenesis of caveolae. The corresponding genes in humans (CAV and CAV2, respectively), have been mapped to a common locus in chromosome 7q31.1, and are possible candidates for the tumor suppressor gene postulated in this region. Here, we show that CAV and CAV2 are independent transcriptional units lying in the same orientation, with CAV2 centromeric and about 17 kb upstream to CAV. The two genes have similar tissue expression patterns. Alternative termination/polyadenylation generates two CAV2 mRNAs. Multiple transcriptional start sites spanning 35 bp upstream from the CAV2 ATG are detected by 5' RACE, consistent with a TATA-less promoter predicted by sequence analysis. The CAV2 promoter region contains two SRE-like boxes resembling those described in the CAV promoter and proposed to link transcription to intracellular cholesterol levels. However, exogenous sterols had only minor effects on CAV and CAV2 RNA levels in HeLa cells, suggesting that SREBPs are not sufficient to regulate caveolin transcription. (C) 2000 Elsevier Science B.V. All rights reserved.
AB - Caveolin-1 and caveolin-2 are related proteins involved in the biogenesis of caveolae. The corresponding genes in humans (CAV and CAV2, respectively), have been mapped to a common locus in chromosome 7q31.1, and are possible candidates for the tumor suppressor gene postulated in this region. Here, we show that CAV and CAV2 are independent transcriptional units lying in the same orientation, with CAV2 centromeric and about 17 kb upstream to CAV. The two genes have similar tissue expression patterns. Alternative termination/polyadenylation generates two CAV2 mRNAs. Multiple transcriptional start sites spanning 35 bp upstream from the CAV2 ATG are detected by 5' RACE, consistent with a TATA-less promoter predicted by sequence analysis. The CAV2 promoter region contains two SRE-like boxes resembling those described in the CAV promoter and proposed to link transcription to intracellular cholesterol levels. However, exogenous sterols had only minor effects on CAV and CAV2 RNA levels in HeLa cells, suggesting that SREBPs are not sufficient to regulate caveolin transcription. (C) 2000 Elsevier Science B.V. All rights reserved.
KW - Caveolae
KW - Cholesterol homeostasis
KW - Sterol responsive element
KW - Tumor suppressor
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UR - http://www.scopus.com/inward/citedby.url?scp=0033975048&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(99)00559-4
DO - 10.1016/S0378-1119(99)00559-4
M3 - Article
C2 - 10675615
AN - SCOPUS:0033975048
VL - 243
SP - 75
EP - 83
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -