Genomic organization of the human metabotropic glutamate receptor subtype 3

Corrado Corti, Cinzia F. Sala, Fentang Yang, Mauro Corsi, John H. Xuereb, Francesco Ferraguti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.

Original languageEnglish
Pages (from-to)207-225
Number of pages19
JournalJournal of Neurogenetics
Volume14
Issue number4
Publication statusPublished - 2000

Fingerprint

Exons
Introns
Genes
Genetic Promoter Regions
Complementary DNA
TATA Box
Polyadenylation
CpG Islands
Genomic Library
5' Flanking Region
Transcription Initiation Site
Consensus Sequence
Fluorescence In Situ Hybridization
Transcription Factors
Chromosomes
Binding Sites
metabotropic glutamate receptor 3

Keywords

  • Chromosome 7
  • Fish
  • Gene
  • mGluR3
  • Transcription initiation

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics
  • Neuroscience(all)

Cite this

Corti, C., Sala, C. F., Yang, F., Corsi, M., Xuereb, J. H., & Ferraguti, F. (2000). Genomic organization of the human metabotropic glutamate receptor subtype 3. Journal of Neurogenetics, 14(4), 207-225.

Genomic organization of the human metabotropic glutamate receptor subtype 3. / Corti, Corrado; Sala, Cinzia F.; Yang, Fentang; Corsi, Mauro; Xuereb, John H.; Ferraguti, Francesco.

In: Journal of Neurogenetics, Vol. 14, No. 4, 2000, p. 207-225.

Research output: Contribution to journalArticle

Corti, C, Sala, CF, Yang, F, Corsi, M, Xuereb, JH & Ferraguti, F 2000, 'Genomic organization of the human metabotropic glutamate receptor subtype 3', Journal of Neurogenetics, vol. 14, no. 4, pp. 207-225.
Corti C, Sala CF, Yang F, Corsi M, Xuereb JH, Ferraguti F. Genomic organization of the human metabotropic glutamate receptor subtype 3. Journal of Neurogenetics. 2000;14(4):207-225.
Corti, Corrado ; Sala, Cinzia F. ; Yang, Fentang ; Corsi, Mauro ; Xuereb, John H. ; Ferraguti, Francesco. / Genomic organization of the human metabotropic glutamate receptor subtype 3. In: Journal of Neurogenetics. 2000 ; Vol. 14, No. 4. pp. 207-225.
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