Abstract
In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.
Original language | English |
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Pages (from-to) | 207-225 |
Number of pages | 19 |
Journal | Journal of Neurogenetics |
Volume | 14 |
Issue number | 4 |
Publication status | Published - 2000 |
Keywords
- Chromosome 7
- Fish
- Gene
- mGluR3
- Transcription initiation
ASJC Scopus subject areas
- Developmental Biology
- Genetics
- Neuroscience(all)