Abstract
In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.
| Original language | English |
|---|---|
| Pages (from-to) | 207-225 |
| Number of pages | 19 |
| Journal | Journal of Neurogenetics |
| Volume | 14 |
| Issue number | 4 |
| Publication status | Published - 2000 |
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Keywords
- Chromosome 7
- Fish
- Gene
- mGluR3
- Transcription initiation
ASJC Scopus subject areas
- Developmental Biology
- Genetics
- Neuroscience(all)
Cite this
Genomic organization of the human metabotropic glutamate receptor subtype 3. / Corti, Corrado; Sala, Cinzia F.; Yang, Fentang; Corsi, Mauro; Xuereb, John H.; Ferraguti, Francesco.
In: Journal of Neurogenetics, Vol. 14, No. 4, 2000, p. 207-225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic organization of the human metabotropic glutamate receptor subtype 3
AU - Corti, Corrado
AU - Sala, Cinzia F.
AU - Yang, Fentang
AU - Corsi, Mauro
AU - Xuereb, John H.
AU - Ferraguti, Francesco
PY - 2000
Y1 - 2000
N2 - In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.
AB - In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.
KW - Chromosome 7
KW - Fish
KW - Gene
KW - mGluR3
KW - Transcription initiation
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M3 - Article
C2 - 11342382
AN - SCOPUS:0034442687
VL - 14
SP - 207
EP - 225
JO - Journal of Neurogenetics
JF - Journal of Neurogenetics
SN - 0167-7063
IS - 4
ER -