Genomic organization of the human metabotropic glutamate receptor subtype 3

Corrado Corti, Cinzia F. Sala, Fentang Yang, Mauro Corsi, John H. Xuereb, Francesco Ferraguti

Research output: Contribution to journalArticle

Abstract

In this study, the genomic organization of the human metabotropic glutamate receptor subtype 3 (mGluR3) gene has been determined. We have identified two transcription initiation sites and the polyadenylation signal by using 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE, respectively. The exon/intron organization of the human mGluR3 gene revealed the presence of 6 exons separated by 5 introns. The size of introns varied from 10.4 to 120 kbp that contained consensus sequences for repetitive elements such as Alu and long interspersed elements. A putative promoter region flanking the 5′ sequence of exon 1 was identified by computer-aided analysis. The putative promoter region was characterized by the presence of a CAAT and GC box, and the absence of a TATA box or CpG islands. Several putative binding sites for transcription factors were also identified. In addition, we have isolated, from a mouse genomic library, part of the mouse mGluR3 gene and found it to correspond to exon 2 in the human mGluR3 gene. The mouse mGluR3 gene was then mapped by fluorescent in situ hybridization analysis to chromosome 5qA2.

Original languageEnglish
Pages (from-to)207-225
Number of pages19
JournalJournal of Neurogenetics
Volume14
Issue number4
Publication statusPublished - 2000

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Keywords

  • Chromosome 7
  • Fish
  • Gene
  • mGluR3
  • Transcription initiation

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics
  • Neuroscience(all)

Cite this

Corti, C., Sala, C. F., Yang, F., Corsi, M., Xuereb, J. H., & Ferraguti, F. (2000). Genomic organization of the human metabotropic glutamate receptor subtype 3. Journal of Neurogenetics, 14(4), 207-225.