Abstract
To facilitate further mutational analysis of NM23-H1 a human metastasis suppressor gene we have established its genomic organization. NM23-H1 is composed of five exons spanning a genomic DNA fragment of 10 kb. Using oligonucleotide primers flanking each exon PCR-SSCP analysis was performed on genomic DNAs of healthy individuals. A common polymorphism a C to T transition was detected 30 nucleotides upstream from the 5' splice site flanking exon 1. As NM23-H1 allele loss and altered expression have been reported in colorectal cancer genomic DNAs of 20 colorectal tumors were analyzed for the presence of gene-specific mutations by PCR-SSCP: no abnormal sequences were detected within the coding and splice site regions of the NM23-H1 gene. This finding suggests that NM23-H1 mutations are rare events in human colorectal cancer.
Original language | English |
---|---|
Pages (from-to) | 2149-2154 |
Number of pages | 6 |
Journal | Anticancer Research |
Volume | 13 |
Issue number | 6 A |
Publication status | Published - 1993 |
Keywords
- Analysis
- Colorectal cancer
- Genomic PCR-SSCP
- Metastasis
- NM23-H1 (NME1) gene
ASJC Scopus subject areas
- Cancer Research
- Oncology