Genomic PCR-SSCP analysis of the metastasis associated NM23-H1(NME1) gene: A study on colorectal cancer

A. Bafico, L. Varesco, L. De Benedetti, M. A. Caligo, V. Gismondi, S. Sciallero, H. Aste, G. B. Ferrara, G. Bevilacqua

Research output: Contribution to journalArticlepeer-review

Abstract

To facilitate further mutational analysis of NM23-H1 a human metastasis suppressor gene we have established its genomic organization. NM23-H1 is composed of five exons spanning a genomic DNA fragment of 10 kb. Using oligonucleotide primers flanking each exon PCR-SSCP analysis was performed on genomic DNAs of healthy individuals. A common polymorphism a C to T transition was detected 30 nucleotides upstream from the 5' splice site flanking exon 1. As NM23-H1 allele loss and altered expression have been reported in colorectal cancer genomic DNAs of 20 colorectal tumors were analyzed for the presence of gene-specific mutations by PCR-SSCP: no abnormal sequences were detected within the coding and splice site regions of the NM23-H1 gene. This finding suggests that NM23-H1 mutations are rare events in human colorectal cancer.

Original languageEnglish
Pages (from-to)2149-2154
Number of pages6
JournalAnticancer Research
Volume13
Issue number6 A
Publication statusPublished - 1993

Keywords

  • Analysis
  • Colorectal cancer
  • Genomic PCR-SSCP
  • Metastasis
  • NM23-H1 (NME1) gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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