Genomic structure and transcriptional regulation of Che-1, a novel partner of Rb

Lucia Monaco, Claudio Passananti, Maurizio Fanciulli

Research output: Contribution to journalArticle

Abstract

We recently identified and cloned a novel human gene, Che-1, whose product interacts with both RNA polymerase II and the retinoblastoma gene product (Rb). Furthermore, we found that Che-1 overexpression counteracts the growth inhibitory effects of Rb, regulating in such way both transcription and cell proliferation. In this paper, we describe the genomic organization of the mouse orthologous Che-1 gene and its promoter region. The promoter is TATA less and presents several potential transcription factor-binding motifs. Importantly, we showed that Che-1 expression is regulated by a negative feedback mechanism, in which this protein is present on its own promoter repressing transcription.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalGene
Volume321
Issue number1-2
DOIs
Publication statusPublished - Dec 4 2003

Keywords

  • ChIP assay
  • Genomic cloning
  • RNA polymerase II subunits

ASJC Scopus subject areas

  • Genetics

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