Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

P. Espinal, E. Nucleo, M. Caltagirone, V. Mattioni Marchetti, M. R. Fernandes, V. Biscaro, R. Rigoli, A. Carattoli, R. Migliavacca, L. Villa

Research output: Contribution to journalArticle


Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenem-resistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying bla NDM-1 or bla OXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying bla OXA-232 and IncF plasmids carrying bla NDM-1 . The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone.

Original languageEnglish
Pages (from-to)385.e1-385.e5
JournalClinical Microbiology and Infection
Issue number3
Publication statusPublished - Mar 2019


  • Antimicrobial resistance
  • Carbapenemase
  • Healthcare-associated infections
  • IncF
  • Outbreak

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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    Espinal, P., Nucleo, E., Caltagirone, M., Mattioni Marchetti, V., Fernandes, M. R., Biscaro, V., Rigoli, R., Carattoli, A., Migliavacca, R., & Villa, L. (2019). Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232. Clinical Microbiology and Infection, 25(3), 385.e1-385.e5.