Genotoxic effects of the carbamate insecticide methomyl. I. In vitro studies with pure compound and the technical formulation 'Lannate 25'

S. Bonatti, C. Bolognesi, P. Degan, A. Abbondandolo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The carbamate insecticide methomyl and the methomyl-containing technical formulation 'Lannate 25' were tested on whole blood human lymphocyte cultures. Both products induced dose-dependent increases in chromosome aberrations and micronuclei. Lannate 25 induced DNA damage as measured by the alkaline elution assay and hydroxylation of guanine at the C8 position. Sister chromatid exchanges were not increased significantly with either product. Overall, the technical formulation was more active than the pure compound, when compared at similar concentrations of active principle. Moreover, a different ratio of CREST-positive/CREST-negative micronuclei was observed with the two products, pure methomyl being relatively more active than Lannate 25 in the induction of CREST-positive micronuclei. On the basis of these results, previous evaluations of methomyl as a nongenotoxic compound should be reconsidered.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalEnvironmental and Molecular Mutagenesis
Volume23
Issue number4
DOIs
Publication statusPublished - 1994

Fingerprint

Methomyl
Carbamates
carbamate (ester)
Insecticides
insecticide
chromosome
blood
assay
DNA
damage
Hydroxylation
Sister Chromatid Exchange
Lymphocytes
Guanine
Chromosomes
In Vitro Techniques
product
effect
Aberrations
Cell culture

Keywords

  • alkaline elution
  • carbamates
  • chromosome aberrations
  • DNA oxidative damage
  • in vitro genotoxicity
  • insecticides
  • methomyl
  • micronuclei
  • pesticides
  • SCE

ASJC Scopus subject areas

  • Genetics
  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology

Cite this

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abstract = "The carbamate insecticide methomyl and the methomyl-containing technical formulation 'Lannate 25' were tested on whole blood human lymphocyte cultures. Both products induced dose-dependent increases in chromosome aberrations and micronuclei. Lannate 25 induced DNA damage as measured by the alkaline elution assay and hydroxylation of guanine at the C8 position. Sister chromatid exchanges were not increased significantly with either product. Overall, the technical formulation was more active than the pure compound, when compared at similar concentrations of active principle. Moreover, a different ratio of CREST-positive/CREST-negative micronuclei was observed with the two products, pure methomyl being relatively more active than Lannate 25 in the induction of CREST-positive micronuclei. On the basis of these results, previous evaluations of methomyl as a nongenotoxic compound should be reconsidered.",
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AU - Bonatti, S.

AU - Bolognesi, C.

AU - Degan, P.

AU - Abbondandolo, A.

PY - 1994

Y1 - 1994

N2 - The carbamate insecticide methomyl and the methomyl-containing technical formulation 'Lannate 25' were tested on whole blood human lymphocyte cultures. Both products induced dose-dependent increases in chromosome aberrations and micronuclei. Lannate 25 induced DNA damage as measured by the alkaline elution assay and hydroxylation of guanine at the C8 position. Sister chromatid exchanges were not increased significantly with either product. Overall, the technical formulation was more active than the pure compound, when compared at similar concentrations of active principle. Moreover, a different ratio of CREST-positive/CREST-negative micronuclei was observed with the two products, pure methomyl being relatively more active than Lannate 25 in the induction of CREST-positive micronuclei. On the basis of these results, previous evaluations of methomyl as a nongenotoxic compound should be reconsidered.

AB - The carbamate insecticide methomyl and the methomyl-containing technical formulation 'Lannate 25' were tested on whole blood human lymphocyte cultures. Both products induced dose-dependent increases in chromosome aberrations and micronuclei. Lannate 25 induced DNA damage as measured by the alkaline elution assay and hydroxylation of guanine at the C8 position. Sister chromatid exchanges were not increased significantly with either product. Overall, the technical formulation was more active than the pure compound, when compared at similar concentrations of active principle. Moreover, a different ratio of CREST-positive/CREST-negative micronuclei was observed with the two products, pure methomyl being relatively more active than Lannate 25 in the induction of CREST-positive micronuclei. On the basis of these results, previous evaluations of methomyl as a nongenotoxic compound should be reconsidered.

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