Genotype-, aging-dependent abnormal caspase activity in Huntington disease blood cells

Research output: Contribution to journalArticlepeer-review

Abstract

Huntington's Disease (HD) is caused by trinucleotide CAG repeat expansion[36 in huntingtin (htt), aprotein with several documented functions. The elongated polyglutamine (polyQ) stretch in the N-terminal region of htt leads to dysfunctional and degenerative events in neurons and peripheral tissues. In this study, by extending the analysis to several caspase activities (i.e. caspase 2, 3, 6, 8 and 9), we describe genotype- and time- dependent caspase activity abnormalities, decreased cell viability and a large set of alterations in mitochondria morphology, in cultured blood cells from HD patients. Patients homozygous for CAG repeat mutations and heterozygous with high size mutations causing juvenile onset (JHD) presented significantly increased caspase 2, 3, 6, 8 and 9 activities, decreased cell viability and pronounced morphological abnormalities, compared with cells carrying low mutation size and controls. After cyanide treatment, all caspases increased their activities in homozygous and highly expanded heterozygous cells, caspase 8 and 9 increased also in those cells carrying low-size mutations, remarking their key role as 'caspase initiators' in HD. The remarkable ageing-dependent abnormalities in peripheral cells carrying particularly toxic mutations (i.e. homozygotes' and JHD'sblood cells) points out the potential dependence of clinical HD development and progression on either mutated htt dosage or missing wild type htt. Peripheral tissues (i.e. blood cells) may theoretically represent an important tool for studying HD mechanisms and searching for new biomarkers, according to the patients' genotype.

Original languageEnglish
Pages (from-to)1599-1607
Number of pages9
JournalJournal of Neural Transmission
Volume118
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Abnormal mitochondria
  • Caspase activities
  • Homozygous CAG mutations
  • Huntingtin (htt) dosage effect
  • Juvenile Huntington disease (JHD)

ASJC Scopus subject areas

  • Biological Psychiatry
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health

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