TY - JOUR
T1 - Genotype of inflammatory cytokines in limbal stem cell graft in Italian patients
AU - Lescai, Francesco
AU - Conti, Luigi
AU - Bartolozzi, Maurizio
AU - Ramazzotti, Giulia
AU - Mazzi, Marta
AU - Sarnicola, Vincenzo
AU - Franceschi, Claudio
PY - 2005/6/24
Y1 - 2005/6/24
N2 - We tested the hypothesis that the genetic capability to mount an inflammatory response might contribute to the inter-individual variability of limbal stem cell graft (LSCG) outcome. Two functional polymorphisms in the IL-6 and TNF-α promoter regions were genotyped in 35 patients. A new score system (clinical assessment score, CAS) was set up in order to classify patients' clinical profile, and the main parameters relevant for LSCG as well as for the follow-up of the patients. Patients carrying at both loci a genotype associated with a lower production of both cytokines were classified as "low producers" (LP), while all the others were classified as "intermediate or high producers" (HP). LP patients did not show any difference in CAS before and after transplantation while a significant difference was present in HP patients. A similar trend was evident in the 35 months of follow-up. Polymorphisms of IL-6 and TNF-α can be used to identify subgroups of patients with higher risk of unsuccessful outcome.
AB - We tested the hypothesis that the genetic capability to mount an inflammatory response might contribute to the inter-individual variability of limbal stem cell graft (LSCG) outcome. Two functional polymorphisms in the IL-6 and TNF-α promoter regions were genotyped in 35 patients. A new score system (clinical assessment score, CAS) was set up in order to classify patients' clinical profile, and the main parameters relevant for LSCG as well as for the follow-up of the patients. Patients carrying at both loci a genotype associated with a lower production of both cytokines were classified as "low producers" (LP), while all the others were classified as "intermediate or high producers" (HP). LP patients did not show any difference in CAS before and after transplantation while a significant difference was present in HP patients. A similar trend was evident in the 35 months of follow-up. Polymorphisms of IL-6 and TNF-α can be used to identify subgroups of patients with higher risk of unsuccessful outcome.
KW - Genetics
KW - IL-6
KW - Inflammation
KW - Limbal stem cell allograft
KW - TNF-α
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U2 - 10.1016/j.bbrc.2005.04.106
DO - 10.1016/j.bbrc.2005.04.106
M3 - Article
C2 - 15896304
AN - SCOPUS:19344366624
VL - 332
SP - 95
EP - 100
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -