Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density

E. Capoluongo, A. Giglio A, M. M. Lavieri, I. Lesnoni-La Parola, C. Ferraro, A. Cristaudo, M. Belardi, F. Leonetti, A. Mastroianni, A. Cambieri, P. Amerio, F. Ameglio

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Staphylococcus aureus strains generally colonize eczematous lesions of subjects with atopic dermatitis much more frequently than in the skin of normal individuals. The aim of this study was to provide a detailed genotypic and phenotypic analysis of S. aureus strains colonizing four different sites (lesional and non-lesional skin areas, nasal and pharyngeal mucosas) of 49 patients with atopic dermatitis. The 88 isolates were analyzed in duplicate by pulsed field gel electrophoresis and in their exfoliative toxin A or B production by latex test. The patients were characterized by age, sex, severity scoring of atopic dermatitis and serum eosinophil cationic protein. Fourteen (28.6%) of the patients were completely negative for S. aureus while 35 (71.4%) were positive in at least one site. The severity scores and eosinophil cationic protein levels were significantly correlated variables (P <0.001), linked to the colonization intensity (P ranging between 0.05 and <0.001 depending on the site) and to the number of colonized sites (P at least <0.01). The genotypic patterns, widely heterogeneous, showed no restriction to peculiar patterns. Only eight strains produced exfoliative toxin B which was significantly restricted to the lesional isolates (P = 0.012).

Original languageEnglish
Pages (from-to)145-155
Number of pages11
JournalJournal of Dermatological Science
Volume26
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Exfoliatins
Atopic Dermatitis
Eosinophil Cationic Protein
Staphylococcus aureus
Skin
Nasal Mucosa
Pulsed Field Gel Electrophoresis
Latex
Electrophoresis
Gels

Keywords

  • Atopic dermatitis
  • ECP
  • Ets
  • PFGE
  • S. aureus
  • SCORAD

ASJC Scopus subject areas

  • Dermatology

Cite this

Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density. / Capoluongo, E.; Giglio A, A.; Lavieri, M. M.; Lesnoni-La Parola, I.; Ferraro, C.; Cristaudo, A.; Belardi, M.; Leonetti, F.; Mastroianni, A.; Cambieri, A.; Amerio, P.; Ameglio, F.

In: Journal of Dermatological Science, Vol. 26, No. 2, 2001, p. 145-155.

Research output: Contribution to journalArticle

Capoluongo, E. ; Giglio A, A. ; Lavieri, M. M. ; Lesnoni-La Parola, I. ; Ferraro, C. ; Cristaudo, A. ; Belardi, M. ; Leonetti, F. ; Mastroianni, A. ; Cambieri, A. ; Amerio, P. ; Ameglio, F. / Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density. In: Journal of Dermatological Science. 2001 ; Vol. 26, No. 2. pp. 145-155.
@article{be05135658b84e41827d6133e4855c8c,
title = "Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density",
abstract = "Staphylococcus aureus strains generally colonize eczematous lesions of subjects with atopic dermatitis much more frequently than in the skin of normal individuals. The aim of this study was to provide a detailed genotypic and phenotypic analysis of S. aureus strains colonizing four different sites (lesional and non-lesional skin areas, nasal and pharyngeal mucosas) of 49 patients with atopic dermatitis. The 88 isolates were analyzed in duplicate by pulsed field gel electrophoresis and in their exfoliative toxin A or B production by latex test. The patients were characterized by age, sex, severity scoring of atopic dermatitis and serum eosinophil cationic protein. Fourteen (28.6{\%}) of the patients were completely negative for S. aureus while 35 (71.4{\%}) were positive in at least one site. The severity scores and eosinophil cationic protein levels were significantly correlated variables (P <0.001), linked to the colonization intensity (P ranging between 0.05 and <0.001 depending on the site) and to the number of colonized sites (P at least <0.01). The genotypic patterns, widely heterogeneous, showed no restriction to peculiar patterns. Only eight strains produced exfoliative toxin B which was significantly restricted to the lesional isolates (P = 0.012).",
keywords = "Atopic dermatitis, ECP, Ets, PFGE, S. aureus, SCORAD",
author = "E. Capoluongo and {Giglio A}, A. and Lavieri, {M. M.} and {Lesnoni-La Parola}, I. and C. Ferraro and A. Cristaudo and M. Belardi and F. Leonetti and A. Mastroianni and A. Cambieri and P. Amerio and F. Ameglio",
year = "2001",
doi = "10.1016/S0923-1811(00)00171-7",
language = "English",
volume = "26",
pages = "145--155",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density

AU - Capoluongo, E.

AU - Giglio A, A.

AU - Lavieri, M. M.

AU - Lesnoni-La Parola, I.

AU - Ferraro, C.

AU - Cristaudo, A.

AU - Belardi, M.

AU - Leonetti, F.

AU - Mastroianni, A.

AU - Cambieri, A.

AU - Amerio, P.

AU - Ameglio, F.

PY - 2001

Y1 - 2001

N2 - Staphylococcus aureus strains generally colonize eczematous lesions of subjects with atopic dermatitis much more frequently than in the skin of normal individuals. The aim of this study was to provide a detailed genotypic and phenotypic analysis of S. aureus strains colonizing four different sites (lesional and non-lesional skin areas, nasal and pharyngeal mucosas) of 49 patients with atopic dermatitis. The 88 isolates were analyzed in duplicate by pulsed field gel electrophoresis and in their exfoliative toxin A or B production by latex test. The patients were characterized by age, sex, severity scoring of atopic dermatitis and serum eosinophil cationic protein. Fourteen (28.6%) of the patients were completely negative for S. aureus while 35 (71.4%) were positive in at least one site. The severity scores and eosinophil cationic protein levels were significantly correlated variables (P <0.001), linked to the colonization intensity (P ranging between 0.05 and <0.001 depending on the site) and to the number of colonized sites (P at least <0.01). The genotypic patterns, widely heterogeneous, showed no restriction to peculiar patterns. Only eight strains produced exfoliative toxin B which was significantly restricted to the lesional isolates (P = 0.012).

AB - Staphylococcus aureus strains generally colonize eczematous lesions of subjects with atopic dermatitis much more frequently than in the skin of normal individuals. The aim of this study was to provide a detailed genotypic and phenotypic analysis of S. aureus strains colonizing four different sites (lesional and non-lesional skin areas, nasal and pharyngeal mucosas) of 49 patients with atopic dermatitis. The 88 isolates were analyzed in duplicate by pulsed field gel electrophoresis and in their exfoliative toxin A or B production by latex test. The patients were characterized by age, sex, severity scoring of atopic dermatitis and serum eosinophil cationic protein. Fourteen (28.6%) of the patients were completely negative for S. aureus while 35 (71.4%) were positive in at least one site. The severity scores and eosinophil cationic protein levels were significantly correlated variables (P <0.001), linked to the colonization intensity (P ranging between 0.05 and <0.001 depending on the site) and to the number of colonized sites (P at least <0.01). The genotypic patterns, widely heterogeneous, showed no restriction to peculiar patterns. Only eight strains produced exfoliative toxin B which was significantly restricted to the lesional isolates (P = 0.012).

KW - Atopic dermatitis

KW - ECP

KW - Ets

KW - PFGE

KW - S. aureus

KW - SCORAD

UR - http://www.scopus.com/inward/record.url?scp=0034992864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034992864&partnerID=8YFLogxK

U2 - 10.1016/S0923-1811(00)00171-7

DO - 10.1016/S0923-1811(00)00171-7

M3 - Article

C2 - 11378332

AN - SCOPUS:0034992864

VL - 26

SP - 145

EP - 155

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 2

ER -