TY - JOUR
T1 - Genotypic, phenotypic and biological characterization of a novel human lung adenocarcinoma cell line (LC 89)
AU - Paganuzz, Michela
AU - Marroni, Paola
AU - Pezzolo, Annalisa
AU - Giinelli, Giorgio
AU - Cavallo, Federico
AU - Pierro, Francesco Di
AU - Angelis, Paolo De
AU - Forni, Guido
AU - Grossi, Carlo E.
PY - 1994
Y1 - 1994
N2 - A cell line termed LC 89 was established from a peritracheal lymph node metastasis removed from a 54-year-old patient who underwent surgery for pulmonary adenocarcinoma. Chromosomal analyses demonstrated structural and numerical aberrations, with a mode of 54 chromosomes per cell and several nonrandom abnormalities. The localization of intermediate filament antigens, low-molecular-weight (LMW) cytokeratins and vimentin, demonstrated a switch from LMW cytokeratins, predominantly expressed in primary tumor cells, to vimentin detected in LC 89 cells that were grown in vitro or transplanted into nude mice. In view of the phenotypic and chromosomal features, LC 89 should provide a useful addition to the cell lines currently available for in vitro and in vivo studies of lung cancer.
AB - A cell line termed LC 89 was established from a peritracheal lymph node metastasis removed from a 54-year-old patient who underwent surgery for pulmonary adenocarcinoma. Chromosomal analyses demonstrated structural and numerical aberrations, with a mode of 54 chromosomes per cell and several nonrandom abnormalities. The localization of intermediate filament antigens, low-molecular-weight (LMW) cytokeratins and vimentin, demonstrated a switch from LMW cytokeratins, predominantly expressed in primary tumor cells, to vimentin detected in LC 89 cells that were grown in vitro or transplanted into nude mice. In view of the phenotypic and chromosomal features, LC 89 should provide a useful addition to the cell lines currently available for in vitro and in vivo studies of lung cancer.
KW - Chromosomal abnormalities in tumor cells
KW - Cytoskeleton of tumor cells
KW - Homogeneous staining regions
KW - Intermediate filaments
KW - Lymph node metastasis
KW - NSCLC human cell lines
KW - Tumor markers
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U2 - 10.1159/000227337
DO - 10.1159/000227337
M3 - Article
C2 - 8196904
AN - SCOPUS:0028209020
VL - 51
SP - 220
EP - 223
JO - Oncology
JF - Oncology
SN - 0030-2414
IS - 3
ER -