Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group

V. Svicher, C. Alteri, M. Montano, A. Nori, R. D'Arrigo, M. Andreoni, G. Angarano, A. Antinori, G. Antonelli, T. Allice, P. Bagnarelli, F. Baldanti, A. Bertoli, M. Borderi, E. Boeri, I. Bon, B. Bruzzone, R. Barresi, S. Calderisi, A. P. CallegaroM. R. Capobianchi, F. Gargiulo, F. Castelli, R. Cauda, F. Ceccherini-Silberstein, M. Clementi, A. Chirianni, M. Colafigli, A. D'Arminio Monforte, A. De Luca, A. Di Biagio, G. Di Nicuolo, G. Di Perri, F. Di Santo, G. Fadda, M. Galli, W. Gennari, V. Ghisetti, A. Costantini, A. Gori, R. Gulminetti, F. Leoncini, G. Maffongelli, F. Maggiolo, R. Maserati, F. Mazzotta, G. Meini, V. Micheli, L. Monno, C. Mussini, S. Nozza, S. Paolucci, G. Palù, S. Parisi, G. Parruti, A. R. Pignataro, T. Quirino, M. C. Re, G. Rizzardini, M. Sanguinetti, R. Santangelo, R. Scaggiante, G. Sterrantino, O. Turriziani, M. L. Vatteroni, C. Viscoli, V. Vullo, M. Zazzi, A. Lazzarin, C. F. Perno

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification. Results: The overall rate of successful V3 sequences ranged from 100% in samples with >3,000 copies HIV-1 DNA/106 PBMCs to 60% in samples with

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalInfection
Volume42
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • CCR5 antagonists
  • Geno2pheno
  • HIV DNA
  • Tropism
  • Virologically suppressed patients

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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