Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group

V. Svicher; C. Alteri; M. Montano; A. Nori; R. D'Arrigo; M. Andreoni; G. Angarano; A. Antinori; G. Antonelli; T. Allice; P. Bagnarelli; F. Baldanti; A. Bertoli; M. Borderi; E. Boeri; I. Bon; B. Bruzzone; R. Barresi; S. Calderisi; A. P. Callegaro; M. R. Capobianchi; F. Gargiulo; F. Castelli; R. Cauda; F. Ceccherini-Silberstein; M. Clementi; A. Chirianni; M. Colafigli; A. D'Arminio Monforte; A. De Luca; A. Di Biagio; G. Di Nicuolo; G. Di Perri; F. Di Santo; G. Fadda; M. Galli; W. Gennari; V. Ghisetti; A. Costantini; A. Gori; R. Gulminetti; F. Leoncini; G. Maffongelli; F. Maggiolo; R. Maserati; F. Mazzotta; G. Meini; V. Micheli; L. Monno; C. Mussini; S. Nozza; S. Paolucci; G. Palù; S. Parisi; G. Parruti; A. R. Pignataro; T. Quirino; M. C. Re; G. Rizzardini; M. Sanguinetti; R. Santangelo; R. Scaggiante; G. Sterrantino; O. Turriziani; M. L. Vatteroni; C. Viscoli; V. Vullo; M. Zazzi; A. Lazzarin; C. F. Perno

doi:10.1007/s15010-013-0510-3

Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group

V. Svicher, C. Alteri, M. Montano, A. Nori, R. D'Arrigo, M. Andreoni, G. Angarano, A. Antinori, G. Antonelli, T. Allice, P. Bagnarelli, F. Baldanti, A. Bertoli, M. Borderi, E. Boeri, I. Bon, B. Bruzzone, R. Barresi, S. Calderisi, A. P. CallegaroM. R. Capobianchi, F. Gargiulo, F. Castelli, R. Cauda, F. Ceccherini-Silberstein, M. Clementi, A. Chirianni, M. Colafigli, A. D'Arminio Monforte, A. De Luca, A. Di Biagio, G. Di Nicuolo, G. Di Perri, F. Di Santo, G. Fadda, M. Galli, W. Gennari, V. Ghisetti, A. Costantini, A. Gori, R. Gulminetti, F. Leoncini, G. Maffongelli, F. Maggiolo, R. Maserati, F. Mazzotta, G. Meini, V. Micheli, L. Monno, C. Mussini, S. Nozza, S. Paolucci, G. Palù, S. Parisi, G. Parruti, A. R. Pignataro, T. Quirino, M. C. Re, G. Rizzardini, M. Sanguinetti, R. Santangelo, R. Scaggiante, G. Sterrantino, O. Turriziani, M. L. Vatteroni, C. Viscoli, V. Vullo, M. Zazzi, A. Lazzarin, C. F. Perno

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification. Results: The overall rate of successful V3 sequences ranged from 100% in samples with >3,000 copies HIV-1 DNA/106 PBMCs to 60% in samples with

Original language	English
Pages (from-to)	61-71
Number of pages	11
Journal	Infection
Volume	42
Issue number	1
DOIs	https://doi.org/10.1007/s15010-013-0510-3
Publication status	Published - 2014

Keywords

CCR5 antagonists
Geno2pheno
HIV DNA
Tropism
Virologically suppressed patients

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1007/s15010-013-0510-3

Cite this

Svicher, V., Alteri, C., Montano, M., Nori, A., D'Arrigo, R., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Allice, T., Bagnarelli, P., Baldanti, F., Bertoli, A., Borderi, M., Boeri, E., Bon, I., Bruzzone, B., Barresi, R., Calderisi, S., ... Perno, C. F. (2014). Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group. Infection, 42(1), 61-71. https://doi.org/10.1007/s15010-013-0510-3

Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice : Results from the DIVA study group. / Svicher, V.; Alteri, C.; Montano, M.; Nori, A.; D'Arrigo, R.; Andreoni, M.; Angarano, G.; Antinori, A.; Antonelli, G.; Allice, T.; Bagnarelli, P.; Baldanti, F.; Bertoli, A.; Borderi, M.; Boeri, E.; Bon, I.; Bruzzone, B.; Barresi, R.; Calderisi, S.; Callegaro, A. P.; Capobianchi, M. R.; Gargiulo, F.; Castelli, F.; Cauda, R.; Ceccherini-Silberstein, F.; Clementi, M.; Chirianni, A.; Colafigli, M.; D'Arminio Monforte, A.; De Luca, A.; Di Biagio, A.; Di Nicuolo, G.; Di Perri, G.; Di Santo, F.; Fadda, G.; Galli, M.; Gennari, W.; Ghisetti, V.; Costantini, A.; Gori, A.; Gulminetti, R.; Leoncini, F.; Maffongelli, G.; Maggiolo, F.; Maserati, R.; Mazzotta, F.; Meini, G.; Micheli, V.; Monno, L.; Mussini, C.; Nozza, S.; Paolucci, S.; Palù, G.; Parisi, S.; Parruti, G.; Pignataro, A. R.; Quirino, T.; Re, M. C.; Rizzardini, G.; Sanguinetti, M.; Santangelo, R.; Scaggiante, R.; Sterrantino, G.; Turriziani, O.; Vatteroni, M. L.; Viscoli, C.; Vullo, V.; Zazzi, M.; Lazzarin, A.; Perno, C. F.

In: Infection, Vol. 42, No. 1, 2014, p. 61-71.

Research output: Contribution to journal › Article › peer-review

Svicher, V, Alteri, C, Montano, M, Nori, A, D'Arrigo, R, Andreoni, M, Angarano, G, Antinori, A, Antonelli, G, Allice, T, Bagnarelli, P, Baldanti, F, Bertoli, A, Borderi, M, Boeri, E, Bon, I, Bruzzone, B, Barresi, R, Calderisi, S, Callegaro, AP, Capobianchi, MR, Gargiulo, F, Castelli, F, Cauda, R, Ceccherini-Silberstein, F, Clementi, M, Chirianni, A, Colafigli, M, D'Arminio Monforte, A, De Luca, A, Di Biagio, A, Di Nicuolo, G, Di Perri, G, Di Santo, F, Fadda, G, Galli, M, Gennari, W, Ghisetti, V, Costantini, A, Gori, A, Gulminetti, R, Leoncini, F, Maffongelli, G, Maggiolo, F, Maserati, R, Mazzotta, F, Meini, G, Micheli, V, Monno, L, Mussini, C, Nozza, S , Paolucci, S, Palù, G, Parisi, S, Parruti, G, Pignataro, AR, Quirino, T, Re, MC, Rizzardini, G, Sanguinetti, M, Santangelo, R, Scaggiante, R, Sterrantino, G, Turriziani, O, Vatteroni, ML, Viscoli, C, Vullo, V, Zazzi, M, Lazzarin, A & Perno, CF 2014, 'Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group', Infection, vol. 42, no. 1, pp. 61-71. https://doi.org/10.1007/s15010-013-0510-3

Svicher, V. ; Alteri, C. ; Montano, M. ; Nori, A. ; D'Arrigo, R. ; Andreoni, M. ; Angarano, G. ; Antinori, A. ; Antonelli, G. ; Allice, T. ; Bagnarelli, P. ; Baldanti, F. ; Bertoli, A. ; Borderi, M. ; Boeri, E. ; Bon, I. ; Bruzzone, B. ; Barresi, R. ; Calderisi, S. ; Callegaro, A. P. ; Capobianchi, M. R. ; Gargiulo, F. ; Castelli, F. ; Cauda, R. ; Ceccherini-Silberstein, F. ; Clementi, M. ; Chirianni, A. ; Colafigli, M. ; D'Arminio Monforte, A. ; De Luca, A. ; Di Biagio, A. ; Di Nicuolo, G. ; Di Perri, G. ; Di Santo, F. ; Fadda, G. ; Galli, M. ; Gennari, W. ; Ghisetti, V. ; Costantini, A. ; Gori, A. ; Gulminetti, R. ; Leoncini, F. ; Maffongelli, G. ; Maggiolo, F. ; Maserati, R. ; Mazzotta, F. ; Meini, G. ; Micheli, V. ; Monno, L. ; Mussini, C. ; Nozza, S. ; Paolucci, S. ; Palù, G. ; Parisi, S. ; Parruti, G. ; Pignataro, A. R. ; Quirino, T. ; Re, M. C. ; Rizzardini, G. ; Sanguinetti, M. ; Santangelo, R. ; Scaggiante, R. ; Sterrantino, G. ; Turriziani, O. ; Vatteroni, M. L. ; Viscoli, C. ; Vullo, V. ; Zazzi, M. ; Lazzarin, A. ; Perno, C. F. / Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice : Results from the DIVA study group. In: Infection. 2014 ; Vol. 42, No. 1. pp. 61-71.

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abstract = "Purpose: We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification. Results: The overall rate of successful V3 sequences ranged from 100% in samples with >3,000 copies HIV-1 DNA/106 PBMCs to 60% in samples with ",

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AU - Alteri, C.

AU - Montano, M.

AU - Nori, A.

AU - D'Arrigo, R.

AU - Andreoni, M.

AU - Angarano, G.

AU - Antinori, A.

AU - Antonelli, G.

AU - Allice, T.

AU - Bagnarelli, P.

AU - Baldanti, F.

AU - Bertoli, A.

AU - Borderi, M.

AU - Boeri, E.

AU - Bon, I.

AU - Bruzzone, B.

AU - Barresi, R.

AU - Calderisi, S.

AU - Callegaro, A. P.

AU - Capobianchi, M. R.

AU - Gargiulo, F.

AU - Castelli, F.

AU - Cauda, R.

AU - Ceccherini-Silberstein, F.

AU - Clementi, M.

AU - Chirianni, A.

AU - Colafigli, M.

AU - D'Arminio Monforte, A.

AU - De Luca, A.

AU - Di Biagio, A.

AU - Di Nicuolo, G.

AU - Di Perri, G.

AU - Di Santo, F.

AU - Fadda, G.

AU - Galli, M.

AU - Gennari, W.

AU - Ghisetti, V.

AU - Costantini, A.

AU - Gori, A.

AU - Gulminetti, R.

AU - Leoncini, F.

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AU - Maserati, R.

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AU - Meini, G.

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AU - Monno, L.

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AU - Parruti, G.

AU - Pignataro, A. R.

AU - Quirino, T.

AU - Re, M. C.

AU - Rizzardini, G.

AU - Sanguinetti, M.

AU - Santangelo, R.

AU - Scaggiante, R.

AU - Sterrantino, G.

AU - Turriziani, O.

AU - Vatteroni, M. L.

AU - Viscoli, C.

AU - Vullo, V.

AU - Zazzi, M.

AU - Lazzarin, A.

AU - Perno, C. F.

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KW - CCR5 antagonists

KW - Geno2pheno

KW - HIV DNA

KW - Tropism

KW - Virologically suppressed patients

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Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group

Abstract

Keywords

ASJC Scopus subject areas

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