Abstract
Purpose: We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification. Results: The overall rate of successful V3 sequences ranged from 100% in samples with >3,000 copies HIV-1 DNA/106 PBMCs to 60% in samples with
Original language | English |
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Pages (from-to) | 61-71 |
Number of pages | 11 |
Journal | Infection |
Volume | 42 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- CCR5 antagonists
- Geno2pheno
- HIV DNA
- Tropism
- Virologically suppressed patients
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases