Genotyping analysis and 18FDG uptake in breast cancer patients: A preliminary research

Valentina Bravatà, Alessandro Stefano, Francesco P. Cammarata, Luigi Minafra, Giorgio Russo, Stefania Nicolosi, Sabina Pulizzi, Cecilia Gelfi, Maria C. Gilardi, Cristina Messa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Diagnostic imaging plays a relevant role in the care of patients with breast cancer (BC). Positron Emission Tomography (PET) with 18F-fluoro-2-deoxy-D-glucose (FDG) has been widely proven to be a clinical tool suitable for BC detection and staging in which the glucose analog supplies metabolic information about the tumor. A limited number of studies, sometimes controversial, describe possible associations between FDG uptake and single nucleotide polymorphisms (SNPs). For this reason this field has to be explored and clarified. We investigated the association of SNPs in GLUT1, HIF-1a, EPAS1, APEX1, VEGFA and MTHFR genes with the FDG uptake in BC. Methods. In 26 caucasian individuals with primary BC, whole-body PET-CT scans were obtained and quantitative analysis was performed by calculating the maximum Standardized Uptake Value normalized to body-weight (SUVmax) and the mean SUV normalized to body-weight corrected for partial volume effect (SUVpvc). Human Gene Mutation Database and dbSNP Short Genetic Variations database were used to analyze gene regions containing the selected SNPs. Patient genotypes were obtained using Sanger DNA sequencing analysis performed by Capillary Electrophoresis. Results: BC patients were genotyped for the following nine SNPs: GLUT1: rs841853 and rs710218; HIF-1a: rs11549465 and rs11549467; EPAS1: rs137853037 and rs137853036; APEX1: rs1130409; VEGFA: rs3025039 and MTHFR: rs1801133. In this work correlations between the nine potentially useful polymorphisms selected and previously suggested with tracer uptake (using both SUVmax and SUVpvc) were not found. Conclusions: The possible functional influence of specific SNPs on FDG uptake needs further studies in human cancer. In summary, this is the first pilot study, to our knowledge, which investigates the association between a large panel of SNPs and FDG uptake specifically in BC patients. This work represents a multidisciplinary and translational medicine approach to study BC where, the possible correlation between SNPs and tracer uptake, may be considered to improve personalized cancer treatment and care.

Original languageEnglish
Article number23
JournalJournal of Experimental and Clinical Cancer Research
Volume32
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Fluorodeoxyglucose F18
Single Nucleotide Polymorphism
Deoxyglucose
Breast Neoplasms
Research
Positron-Emission Tomography
Body Weight
Genes
Genetic Databases
Neoplasms
Translational Medical Research
Neoplasm Staging
Capillary Electrophoresis
Diagnostic Imaging
DNA Sequence Analysis
Patient Care
Genotype
Databases
Glucose
Mutation

Keywords

  • Breast cancer
  • PET-CT
  • Single nucleotide polymorphisms
  • SUVmax
  • SUVpvc

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Bravatà, V., Stefano, A., Cammarata, F. P., Minafra, L., Russo, G., Nicolosi, S., ... Messa, C. (2013). Genotyping analysis and 18FDG uptake in breast cancer patients: A preliminary research. Journal of Experimental and Clinical Cancer Research, 32(1), [23]. https://doi.org/10.1186/1756-9966-32-23

Genotyping analysis and 18FDG uptake in breast cancer patients : A preliminary research. / Bravatà, Valentina; Stefano, Alessandro; Cammarata, Francesco P.; Minafra, Luigi; Russo, Giorgio; Nicolosi, Stefania; Pulizzi, Sabina; Gelfi, Cecilia; Gilardi, Maria C.; Messa, Cristina.

In: Journal of Experimental and Clinical Cancer Research, Vol. 32, No. 1, 23, 2013.

Research output: Contribution to journalArticle

Bravatà, V, Stefano, A, Cammarata, FP, Minafra, L, Russo, G, Nicolosi, S, Pulizzi, S, Gelfi, C, Gilardi, MC & Messa, C 2013, 'Genotyping analysis and 18FDG uptake in breast cancer patients: A preliminary research', Journal of Experimental and Clinical Cancer Research, vol. 32, no. 1, 23. https://doi.org/10.1186/1756-9966-32-23
Bravatà, Valentina ; Stefano, Alessandro ; Cammarata, Francesco P. ; Minafra, Luigi ; Russo, Giorgio ; Nicolosi, Stefania ; Pulizzi, Sabina ; Gelfi, Cecilia ; Gilardi, Maria C. ; Messa, Cristina. / Genotyping analysis and 18FDG uptake in breast cancer patients : A preliminary research. In: Journal of Experimental and Clinical Cancer Research. 2013 ; Vol. 32, No. 1.
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AU - Russo, Giorgio

AU - Nicolosi, Stefania

AU - Pulizzi, Sabina

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