Genotyping of circulating measles strains in Italy in 2010

Melissa Baggieri, Claudia Fortuna, Filippo Ansaldi, Maria Chironna, Antonella Marchi, Paola Bucci, Eleonora Benedetti, Martina Del Manso, Silvia Declich, Loredana Nicoletti, Fabio Magurano

Research output: Contribution to journalArticle

Abstract

Introduction. The European Regional Office of the World Health Organization developed a strategic approach to stop the indigenous transmission of measles in its 53 Member States by 2015. In Italy, laboratory surveillance activity is implemented by the National Reference Laboratory for Measles and Rubella at the Italian National Institute of Health (Istituto Superiore di Sanità, Rome). The role of the National Reference Laboratory is to strengthen surveillance systems through rigorous case investigation and laboratory confirmation of suspected sporadic cases and outbreaks. Genetic characterization of wild-type measles virus is an essential component of the laboratorybased surveillance. This study describes the molecular characterization of measles virus strains isolated during 2010. Methods. Dried blood spots, urine and oral fluid samples were collected from patients with a suspected measles infection. Serological tests were performed on capillary blood, and viral detection was performed on urine and oral fluid samples through molecular assay. Positive samples were sequenced and phylogenetically analysed. Results and discussion. The phylogenetic analysis showed a co-circulation of genotypes D4 and D8, and sporadic cases associated to genotypes D9 and B3. Then, molecular epidemiology of measles cases permitted to establish that D4 and D8 were the endemic genotypes in Italy during 2010.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalAnnali dell'Istituto Superiore di Sanita
Volume50
Issue number4
DOIs
Publication statusPublished - 2014

Keywords

  • Genotype
  • Measles
  • Molecular epidemiology
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Fingerprint Dive into the research topics of 'Genotyping of circulating measles strains in Italy in 2010'. Together they form a unique fingerprint.

  • Cite this

    Baggieri, M., Fortuna, C., Ansaldi, F., Chironna, M., Marchi, A., Bucci, P., Benedetti, E., Del Manso, M., Declich, S., Nicoletti, L., & Magurano, F. (2014). Genotyping of circulating measles strains in Italy in 2010. Annali dell'Istituto Superiore di Sanita, 50(4), 345-350. https://doi.org/10.4415/ANN-14-04-09