Genotyping reveals a wide heterogeneity of Tropheryma whipplei

Wenjun Li, Florence Fenollar, Jean Marc Rolain, Pierre Edouard Fournier, Gerhard E. Feurle, Christian Müller, Verena Moos, Thomas Marth, Martin Altwegg, Romana C. Calligaris-Maibach, Thomas Schneider, Federico Biagi, Bernard La Scola, Didier Raoult

Research output: Contribution to journalArticlepeer-review


Tropheryma whipplei, the causative agent of Whipple's disease, is associated with various clinical manifestations as well as an asymptomatic carrier status, and it exhibits genetic heterogeneity. However, relationships that may exist between environmental and clinical strains are unknown. Herein, we developed an efficient genotyping system based on four highly variable genomic sequences (HVGSs) selected on the basis of genome comparison. We analysed 39 samples from 39 patients with Whipple's disease and 10 samples from 10 asymptomatic carriers. Twenty-six classic gastrointestinal Whipple's disease associated with additional mandestations, six relapses of classic Whipple's disease (three gastrointestinal and three neurological relapses), and seven isolated infections due to T. whipplei without digestive involvement (five endocarditis, one spondylodiscitis and one neurological infection) were included in the study. We identified 24 HVGS genotypes among 39 T. whipplei DNA samples from the patients and 10 T. whipplei DNA samples from the asymptomatic carriers. No significant correlation between HVGS genotypes and clinical manifestations of Whipple's diseae, or asymptomatic carriers, was found for the 49 samples tested. Our observations revealed a high genetic diversity of T. whipplei strains that is apparently independent of geographical distribution and unrelated to bacterial pathogenicity. Genotyping in Whipple's disease may, however, be useful in epidemiological studies.

Original languageEnglish
Pages (from-to)521-527
Number of pages7
Issue number2
Publication statusPublished - Feb 2008

ASJC Scopus subject areas

  • Microbiology


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