TY - JOUR
T1 - Genotyping Reveals High Clonal Diversity and Widespread Genotypes of Candida Causing Candidemia at Distant Geographical Areas
AU - Guinea, Jesús
AU - Arendrup, Maiken C.
AU - Cantón, Rafael
AU - Cantón, Emilia
AU - García-Rodríguez, Julio
AU - Gómez, Ana
AU - de la Pedrosa, Elia Gómez G.
AU - Hare, Rasmus K.
AU - Orden, Beatriz
AU - Sanguinetti, Maurizio
AU - Pemán, Javier
AU - Posteraro, Brunella
AU - Ruiz-Gaitán, Alba
AU - Parisi, Gabriella
AU - Da Matta, Daniel Archimedes
AU - Colombo, Arnaldo L.
AU - Sánchez-Carrillo, Carlos
AU - Reigadas, Elena
AU - Muñoz, Patricia
AU - Escribano, Pilar
N1 - Funding Information:
Wu, Y., Zhou, H. J., Che, J., Li, W. G., Bian, F. N., Yu, S. B., et al. (2014). Multilocus microsatellite markers for molecular typing of Candida tropicalis isolates. BMC Microbiol. 14:245. doi: 10.1186/s12866-014-0245-z Conflict of Interest: JG has received funds for participating at educational activities organized on behalf of Astellas, Gilead, MSD, Scynexis, and Biotoscana-United Medical; he has also received research funds from FIS, Gilead, Scynexis, and Cidara, outside the submitted work. AC received educational grants from Biotoscana-United Medical, MSD, Pfizer and research grant from Astellas and Pfizer. MA reports personal fees from Astellas, Basilea, Gilead, MSD, Pfizer, T2Biosystems, and Novartis, other from Astellas, Basilea, Gilead, T2Biosystems, F2G, Novabiotics and Amplyx outside the submitted work.
Funding Information:
This work was supported by grants CP15/00115 and PI16/01012 from the Fondo de Investigación Sanitaria (FIS. Instituto de Salud Carlos III; Plan Nacional de I+D+I 2013–2016). This study was co-funded by the European Regional Development Fund (FEDER) A way of making Europe. In Brazil, this work was
Funding Information:
The authors are grateful to Ciencia Traducida for editing and proofreading assistance. Funding. This work was supported by grants CP15/00115 and PI16/01012 from the Fondo de Investigaci?n Sanitaria (FIS. Instituto de Salud Carlos III; Plan Nacional de I+D+I 2013?2016). This study was co-funded by the European Regional Development Fund (FEDER) A way of making Europe. In Brazil, this work was supported by a grant 2017/02203-7 from Funda??o de Amparo a Pesquisa-S?o Paulo (FAPESP). The funders had no role in the study design, data collection, analysis, decision to publish, or preparation/content of the manuscript. PE (MSI15/00115) and JG (MSII15/00006) are recipients of a Miguel Servet contract supported by the FIS.
Publisher Copyright:
© Copyright © 2020 Guinea, Arendrup, Cantón, Cantón, García-Rodríguez, Gómez, de la Pedrosa, Hare, Orden, Sanguinetti, Pemán, Posteraro, Ruiz-Gaitán, Parisi, Da Matta, Colombo, Sánchez-Carrillo, Reigadas, Muñoz and Escribano.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/5
Y1 - 2020/5/5
N2 - The objectives of this study were to gain further insight on Candida genotype distribution and percentage of clustered isolates between hospitals and to identify potential clusters involving different hospitals and cities. We aim to genotype Candida spp. isolates causing candidemia in patients admitted to 16 hospitals in Spain, Italy, Denmark, and Brazil. Eight hundred and eighty-four isolates (Candida albicans, n = 534; C. parapsilosis, n = 282; and C. tropicalis, n = 68) were genotyped using species-specific microsatellite markers. CDC3, EF3, HIS3, CAI, CAIII, and CAVI were used for C. albicans, Ctrm1, Ctrm10, Ctrm12, Ctrm21, Ctrm24, and Ctrm28 for C. tropicalis, and CP1, CP4a, CP6, and B for C. parapsilosis. Genotypes were classified as singletons (genotype only found once) or clusters (same genotype infecting two or more patients). Clusters were defined as intra-hospital (involving patients admitted to a single hospital), intra-ward (involving patients admitted to the same hospital ward) or widespread (involving patients admitted to different hospitals). The percentage of clusters and the proportion of patients involved in clusters among species, genotypic diversity and distribution of genetic diversity were assessed. Seven hundred and twenty-three genotypes were detected, 78 (11%) being clusters, most of which (57.7%; n = 45/78) were intra-hospital clusters including intra-ward ones (42.2%; n = 19/45). The proportion of clusters was not statistically different between species, but the percentage of patients in clusters varied among hospitals. A number of genotypes (7.2%; 52/723) were widespread (found at different hospitals), comprising 66.7% (52/78) of clusters, and involved patients at hospitals in the same city (n = 21) or in different cities (n = 31). Only one C. parapsilosis cluster was a widespread genotype found in all four countries. Around 11% of C. albicans and C. parapsilosis isolates causing candidemia are clusters that may result from patient-to-patient transmission, widespread genotypes commonly found in unrelated patients, or insufficient microsatellite typing genetic discrimination.
AB - The objectives of this study were to gain further insight on Candida genotype distribution and percentage of clustered isolates between hospitals and to identify potential clusters involving different hospitals and cities. We aim to genotype Candida spp. isolates causing candidemia in patients admitted to 16 hospitals in Spain, Italy, Denmark, and Brazil. Eight hundred and eighty-four isolates (Candida albicans, n = 534; C. parapsilosis, n = 282; and C. tropicalis, n = 68) were genotyped using species-specific microsatellite markers. CDC3, EF3, HIS3, CAI, CAIII, and CAVI were used for C. albicans, Ctrm1, Ctrm10, Ctrm12, Ctrm21, Ctrm24, and Ctrm28 for C. tropicalis, and CP1, CP4a, CP6, and B for C. parapsilosis. Genotypes were classified as singletons (genotype only found once) or clusters (same genotype infecting two or more patients). Clusters were defined as intra-hospital (involving patients admitted to a single hospital), intra-ward (involving patients admitted to the same hospital ward) or widespread (involving patients admitted to different hospitals). The percentage of clusters and the proportion of patients involved in clusters among species, genotypic diversity and distribution of genetic diversity were assessed. Seven hundred and twenty-three genotypes were detected, 78 (11%) being clusters, most of which (57.7%; n = 45/78) were intra-hospital clusters including intra-ward ones (42.2%; n = 19/45). The proportion of clusters was not statistically different between species, but the percentage of patients in clusters varied among hospitals. A number of genotypes (7.2%; 52/723) were widespread (found at different hospitals), comprising 66.7% (52/78) of clusters, and involved patients at hospitals in the same city (n = 21) or in different cities (n = 31). Only one C. parapsilosis cluster was a widespread genotype found in all four countries. Around 11% of C. albicans and C. parapsilosis isolates causing candidemia are clusters that may result from patient-to-patient transmission, widespread genotypes commonly found in unrelated patients, or insufficient microsatellite typing genetic discrimination.
KW - Candida
KW - cluster
KW - genotyping
KW - microsatellite
KW - widespread
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U2 - 10.3389/fcimb.2020.00166
DO - 10.3389/fcimb.2020.00166
M3 - Article
C2 - 32432048
AN - SCOPUS:85084962762
VL - 10
JO - Frontiers in cellular and infection microbiology
JF - Frontiers in cellular and infection microbiology
SN - 2235-2988
M1 - 166
ER -