GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex

Jinte Middeldorp, Karin Boer, Jacqueline A. Sluijs, Lidia De Filippis, Férechté Encha-Razavi, Angelo L. Vescovi, Dick F. Swaab, Eleonora Aronica, Elly M. Hol

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A subpopulation of glial fibrillary acidic protein (GFAP)-expressing cells located along the length of the lateral ventricles in the subventricular zone (SVZ) have been identified as the multipotent neural stem cells of the adult mammalian brain. We have previously found that, in the adult human brain, a splice variant of GFAP, termed GFAPδ, was expressed specifically in these cells. To investigate whether GFAPd is also present in the precursors of SVZ astrocytes during development and whether GFAPδ could play a role in the developmental process, we analyzed GFAPδ expression in the normal developing human cortex and in the cortex of foetuses with the migration disorder lissencephaly type II. We demonstrated for the first time that GFAPδ is specifically expressed in radial glia and SVZ neural progenitors during human brain development. Expression of GFAPδ in radial glia starts at around 13 weeks of pregnancy and disappears before birth. GFAPδ is continuously expressed in the SVZ progenitors at later gestational ages and in the postnatal brain. Co-localization with Ki67 proved that these GFAPδ-expressing cells are able to proliferate. Furthermore, we showed that the expression pattern of GFAPδ was disturbed in lissencephaly type II. Overall, these results suggest that the adult SVZ is indeed a remnant of the foetal SVZ, which develops from radial glia. Furthermore, we provide evidence that GFAPδ can distinguish resting astrocytes from proliferating SVZ progenitors.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalDevelopment
Volume137
Issue number2
DOIs
Publication statusPublished - Jan 15 2010

Fingerprint

Lateral Ventricles
Glial Fibrillary Acidic Protein
Neuroglia
Lissencephaly
Brain
Astrocytes
Multipotent Stem Cells
Neural Stem Cells
Human Development
Gestational Age
Fetus
Parturition
Pregnancy

Keywords

  • GFAP
  • Human brain development
  • Lissencephaly
  • Neural progenitors
  • Radial glia

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Middeldorp, J., Boer, K., Sluijs, J. A., De Filippis, L., Encha-Razavi, F., Vescovi, A. L., ... Hol, E. M. (2010). GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex. Development, 137(2), 313-321. https://doi.org/10.1242/dev.041632

GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex. / Middeldorp, Jinte; Boer, Karin; Sluijs, Jacqueline A.; De Filippis, Lidia; Encha-Razavi, Férechté; Vescovi, Angelo L.; Swaab, Dick F.; Aronica, Eleonora; Hol, Elly M.

In: Development, Vol. 137, No. 2, 15.01.2010, p. 313-321.

Research output: Contribution to journalArticle

Middeldorp, J, Boer, K, Sluijs, JA, De Filippis, L, Encha-Razavi, F, Vescovi, AL, Swaab, DF, Aronica, E & Hol, EM 2010, 'GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex', Development, vol. 137, no. 2, pp. 313-321. https://doi.org/10.1242/dev.041632
Middeldorp J, Boer K, Sluijs JA, De Filippis L, Encha-Razavi F, Vescovi AL et al. GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex. Development. 2010 Jan 15;137(2):313-321. https://doi.org/10.1242/dev.041632
Middeldorp, Jinte ; Boer, Karin ; Sluijs, Jacqueline A. ; De Filippis, Lidia ; Encha-Razavi, Férechté ; Vescovi, Angelo L. ; Swaab, Dick F. ; Aronica, Eleonora ; Hol, Elly M. / GFAPδ in radial glia and subventricular zone progenitors in the developing human cortex. In: Development. 2010 ; Vol. 137, No. 2. pp. 313-321.
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