Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo

Maria Teresa Conconi, Beatrice Nico, Diego Guidolin, Silvia Baiguera, Raffaella Spinazzi, Piera Rebuffat, Ludwik K. Malendowicz, Angelo Vacca, Gianni Carraro, Pier Paolo Parnigotto, Gastone G. Nussdorfer, Domenico Ribatti

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Recent evidence indicates that ghrelin, an endogenous ligand of the growth hormone secretagogue receptor (GHS-R), is highly expressed in the cardiovascular system, and in this study we addressed the possibility that ghrelin may affect angiogenesis in vitro and in vivo. Reverse transcription-polymerase chain reaction showed that human umbilical vein endothelial cells (HUVECs) express ghrelin and GHS-R mRNAs. Ghrelin inhibited FGF-2-induced proliferation of HUVECs cultured in vitro, the maximal effective concentration being 10 -8 M, and this effect was annulled by the GHS-R antagonist d-Lys 3-growth hormone releasing peptide-6. FGF-2 stimulated HUVEC cultured on Matrigel to form capillary-like structures, and ghrelin (10 -8 M) suppressed this effect. In the chick embryo chorioallantoic membrane in vivo assay, FGF-2 induced a strong angiogenic response, which was counteracted by ghrelin (500 ng). Taken together, these findings suggest that ghrelin acts as an angiostatic molecule and indicate that its activity is comparable to that of a well-known angiostatic agent, i.e., vinblastine. The antiangiogenic activity of ghrelin deserves further investigations, alone or together with other antiangiogenic agents, for the treatment of pathological conditions characterized by enhanced angiogenesis.

Original languageEnglish
Pages (from-to)2179-2185
Number of pages7
JournalPeptides
Volume25
Issue number12
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Ghrelin
Fibroblast Growth Factor 2
Ghrelin Receptor
Endothelial cells
Human Umbilical Vein Endothelial Cells
Angiogenesis Inhibitors
Chorioallantoic Membrane
Cardiovascular system
Vinblastine
In Vitro Techniques
Polymerase chain reaction
Chick Embryo
Transcription
Cardiovascular System
Reverse Transcription
Assays
Ligands
Membranes
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Angiogenesis
  • Antiangiogenesis
  • FGF-2
  • Ghrelin
  • Vinblastine

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Conconi, M. T., Nico, B., Guidolin, D., Baiguera, S., Spinazzi, R., Rebuffat, P., ... Ribatti, D. (2004). Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo. Peptides, 25(12), 2179-2185. https://doi.org/10.1016/j.peptides.2004.08.011

Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo. / Conconi, Maria Teresa; Nico, Beatrice; Guidolin, Diego; Baiguera, Silvia; Spinazzi, Raffaella; Rebuffat, Piera; Malendowicz, Ludwik K.; Vacca, Angelo; Carraro, Gianni; Parnigotto, Pier Paolo; Nussdorfer, Gastone G.; Ribatti, Domenico.

In: Peptides, Vol. 25, No. 12, 12.2004, p. 2179-2185.

Research output: Contribution to journalArticle

Conconi, MT, Nico, B, Guidolin, D, Baiguera, S, Spinazzi, R, Rebuffat, P, Malendowicz, LK, Vacca, A, Carraro, G, Parnigotto, PP, Nussdorfer, GG & Ribatti, D 2004, 'Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo', Peptides, vol. 25, no. 12, pp. 2179-2185. https://doi.org/10.1016/j.peptides.2004.08.011
Conconi MT, Nico B, Guidolin D, Baiguera S, Spinazzi R, Rebuffat P et al. Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo. Peptides. 2004 Dec;25(12):2179-2185. https://doi.org/10.1016/j.peptides.2004.08.011
Conconi, Maria Teresa ; Nico, Beatrice ; Guidolin, Diego ; Baiguera, Silvia ; Spinazzi, Raffaella ; Rebuffat, Piera ; Malendowicz, Ludwik K. ; Vacca, Angelo ; Carraro, Gianni ; Parnigotto, Pier Paolo ; Nussdorfer, Gastone G. ; Ribatti, Domenico. / Ghrelin inhibits FGF-2-mediated angiogenesis in vitro and in vivo. In: Peptides. 2004 ; Vol. 25, No. 12. pp. 2179-2185.
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