Giant cell formation in Hodgkin's disease

G. Sitar, A. Bianchi Santamaria, V. Rosti, P. Shaskin, R. Blago, L. Santamaria, E. Ascari

Research output: Contribution to journalArticle

Abstract

The identity of Reed-Sternberg cells in Hodgkin's disease has remained an unresolved issue, though many studies have addressed this question. Giant cells are usually formed either by endomitosis without cytoplasmic division or by cell fusion through cytokines or viruses. Growing evidence associates Epstein-Barr virus (EBV) with Hodgkin's disease, a major issue being whether EBV is a passenger virus or has an aetiological role. This communication describes experimental conditions enabling observation of giant cell cytogenesis from peripheral blood mononuclear cells in culture. Mononuclear cells were isolated from autologous peripheral blood and cocultured with a single-cell suspension obtained from Hodgkin's lymph nodes in a culture chamber where the two cell populations are isolated by a microporous membrane that allows only cytokines and viruses to pass through. Under these experimental conditions, giant cells are formed in the peripheral blood mononuclear cell fraction; some of them appear morphologically indistinguishable from Reed-Sternberg cells and their mononuclear variant, while others much resemble Langhans giant cells. Some of these giant cells are positive for EBV DNA by in situ hybridization. These results suggest that an EBV-dependent biological activity is responsible for giant cell cytogenesis originating from lymphocytes and monocytes, induced either by EBV and/or cytokines.

Original languageEnglish
Pages (from-to)499-515
Number of pages17
JournalResearch in Immunology
Volume145
Issue number7
DOIs
Publication statusPublished - 1994

Fingerprint

Giant Cells
Human Herpesvirus 4
Hodgkin Disease
Reed-Sternberg Cells
Cytokines
Viruses
Blood Cells
Langhans Giant Cells
Cytokinesis
Cell Fusion
In Situ Hybridization
Monocytes
Suspensions
Cell Culture Techniques
Lymph Nodes
Observation
Lymphocytes
Membranes
DNA
Population

Keywords

  • Cell fusion Reed-Sternberg cell
  • Cellular identity.
  • EBV
  • Experimental study
  • Hodgkin's disease

ASJC Scopus subject areas

  • Immunology

Cite this

Sitar, G., Santamaria, A. B., Rosti, V., Shaskin, P., Blago, R., Santamaria, L., & Ascari, E. (1994). Giant cell formation in Hodgkin's disease. Research in Immunology, 145(7), 499-515. https://doi.org/10.1016/S0923-2494(94)80069-3

Giant cell formation in Hodgkin's disease. / Sitar, G.; Santamaria, A. Bianchi; Rosti, V.; Shaskin, P.; Blago, R.; Santamaria, L.; Ascari, E.

In: Research in Immunology, Vol. 145, No. 7, 1994, p. 499-515.

Research output: Contribution to journalArticle

Sitar, G, Santamaria, AB, Rosti, V, Shaskin, P, Blago, R, Santamaria, L & Ascari, E 1994, 'Giant cell formation in Hodgkin's disease', Research in Immunology, vol. 145, no. 7, pp. 499-515. https://doi.org/10.1016/S0923-2494(94)80069-3
Sitar G, Santamaria AB, Rosti V, Shaskin P, Blago R, Santamaria L et al. Giant cell formation in Hodgkin's disease. Research in Immunology. 1994;145(7):499-515. https://doi.org/10.1016/S0923-2494(94)80069-3
Sitar, G. ; Santamaria, A. Bianchi ; Rosti, V. ; Shaskin, P. ; Blago, R. ; Santamaria, L. ; Ascari, E. / Giant cell formation in Hodgkin's disease. In: Research in Immunology. 1994 ; Vol. 145, No. 7. pp. 499-515.
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