Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois

Claudio De Liberato, Federica Berrilli, Marianna Marangi, Maristella Santoro, Tiziana Trogu, Lorenza Putignani, Paolo Lanfranchi, Francesco Ferretti, Stefano D'Amelio, Annunziata Giangaspero

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.

Original languageEnglish
Article number650
JournalParasites and Vectors
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 21 2015

Fingerprint

Rupicapra
Giardia lamblia
Cysts
Genotype
Zoonoses
Feces
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction

Keywords

  • end-point-PCR
  • Giardia duodenalis
  • IF
  • qPCR
  • Rupicapra pyrenaica ornata
  • Rupicapra rupicapra rupicapra

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois. / De Liberato, Claudio; Berrilli, Federica; Marangi, Marianna; Santoro, Maristella; Trogu, Tiziana; Putignani, Lorenza; Lanfranchi, Paolo; Ferretti, Francesco; D'Amelio, Stefano; Giangaspero, Annunziata.

In: Parasites and Vectors, Vol. 8, No. 1, 650, 21.12.2015.

Research output: Contribution to journalArticle

De Liberato, C, Berrilli, F, Marangi, M, Santoro, M, Trogu, T, Putignani, L, Lanfranchi, P, Ferretti, F, D'Amelio, S & Giangaspero, A 2015, 'Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois', Parasites and Vectors, vol. 8, no. 1, 650. https://doi.org/10.1186/s13071-015-1243-1
De Liberato, Claudio ; Berrilli, Federica ; Marangi, Marianna ; Santoro, Maristella ; Trogu, Tiziana ; Putignani, Lorenza ; Lanfranchi, Paolo ; Ferretti, Francesco ; D'Amelio, Stefano ; Giangaspero, Annunziata. / Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois. In: Parasites and Vectors. 2015 ; Vol. 8, No. 1.
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AU - Berrilli, Federica

AU - Marangi, Marianna

AU - Santoro, Maristella

AU - Trogu, Tiziana

AU - Putignani, Lorenza

AU - Lanfranchi, Paolo

AU - Ferretti, Francesco

AU - D'Amelio, Stefano

AU - Giangaspero, Annunziata

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