Abstract
Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.
| Original language | English |
|---|---|
| Article number | 650 |
| Journal | Parasites and Vectors |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 21 2015 |
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Keywords
- end-point-PCR
- Giardia duodenalis
- IF
- qPCR
- Rupicapra pyrenaica ornata
- Rupicapra rupicapra rupicapra
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois. / De Liberato, Claudio; Berrilli, Federica; Marangi, Marianna; Santoro, Maristella; Trogu, Tiziana; Putignani, Lorenza; Lanfranchi, Paolo; Ferretti, Francesco; D'Amelio, Stefano; Giangaspero, Annunziata.
In: Parasites and Vectors, Vol. 8, No. 1, 650, 21.12.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
AU - De Liberato, Claudio
AU - Berrilli, Federica
AU - Marangi, Marianna
AU - Santoro, Maristella
AU - Trogu, Tiziana
AU - Putignani, Lorenza
AU - Lanfranchi, Paolo
AU - Ferretti, Francesco
AU - D'Amelio, Stefano
AU - Giangaspero, Annunziata
PY - 2015/12/21
Y1 - 2015/12/21
N2 - Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.
AB - Background: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. Methods: A total of 157 fecal samples from Alpine Rupicapra rupicapra rupicapra and Apennine Rupicapra pyrenaica ornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. Results: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. Conclusions: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.
KW - end-point-PCR
KW - Giardia duodenalis
KW - IF
KW - qPCR
KW - Rupicapra pyrenaica ornata
KW - Rupicapra rupicapra rupicapra
UR - http://www.scopus.com/inward/record.url?scp=84955421922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955421922&partnerID=8YFLogxK
U2 - 10.1186/s13071-015-1243-1
DO - 10.1186/s13071-015-1243-1
M3 - Article
AN - SCOPUS:84955421922
VL - 8
JO - Parasites and Vectors
JF - Parasites and Vectors
SN - 1756-3305
IS - 1
M1 - 650
ER -