Abstract
Gut-enriched Kruppel-like factor (GKLF) is a transcriptional regulator expressed in differentiated epithelia. We identified GKLF transcript as a regulated element in thymic epithelium of recombinase-deficient mice during thymus development induced by anti-CD3 antibody injection. This treatment recapitulates the organogenetic process depending on productive rearrangement of T cell receptor (TCR) β gene with thymocytes expansion and acquisition of the CD4+8+ double positive phenotype. In wildtype mice, GKLF is expressed very early in embryogenesis and becomes intensely up-regulated in thymus epithelium at day 18 of gestation when TCR β expressing cells have selectively expanded and express both CD4 and CD8. The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR β gene rearrangement. Furthermore, GKLF expression in hematopoietic stroma might suggest the thus far uncharacterised participation of this factor in hematopoiesis.
| Original language | English |
|---|---|
| Pages (from-to) | 103-113 |
| Number of pages | 11 |
| Journal | Mechanisms of Development |
| Volume | 81 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Mar 1 1999 |
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Keywords
- Gene regulation
- Hematopoiesis
- Stromal cells
- Thymus
- Transcription factors
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk. / Panigada, Maddalena; Porcellini, Simona; Sutti, Francesca; Doneda, Luisa; Pozzoli, Ombretta; Consalez, G. Giacomo; Guttinger, Maria; Grassi, Fabio.
In: Mechanisms of Development, Vol. 81, No. 1-2, 01.03.1999, p. 103-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk
AU - Panigada, Maddalena
AU - Porcellini, Simona
AU - Sutti, Francesca
AU - Doneda, Luisa
AU - Pozzoli, Ombretta
AU - Consalez, G. Giacomo
AU - Guttinger, Maria
AU - Grassi, Fabio
PY - 1999/3/1
Y1 - 1999/3/1
N2 - Gut-enriched Kruppel-like factor (GKLF) is a transcriptional regulator expressed in differentiated epithelia. We identified GKLF transcript as a regulated element in thymic epithelium of recombinase-deficient mice during thymus development induced by anti-CD3 antibody injection. This treatment recapitulates the organogenetic process depending on productive rearrangement of T cell receptor (TCR) β gene with thymocytes expansion and acquisition of the CD4+8+ double positive phenotype. In wildtype mice, GKLF is expressed very early in embryogenesis and becomes intensely up-regulated in thymus epithelium at day 18 of gestation when TCR β expressing cells have selectively expanded and express both CD4 and CD8. The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR β gene rearrangement. Furthermore, GKLF expression in hematopoietic stroma might suggest the thus far uncharacterised participation of this factor in hematopoiesis.
AB - Gut-enriched Kruppel-like factor (GKLF) is a transcriptional regulator expressed in differentiated epithelia. We identified GKLF transcript as a regulated element in thymic epithelium of recombinase-deficient mice during thymus development induced by anti-CD3 antibody injection. This treatment recapitulates the organogenetic process depending on productive rearrangement of T cell receptor (TCR) β gene with thymocytes expansion and acquisition of the CD4+8+ double positive phenotype. In wildtype mice, GKLF is expressed very early in embryogenesis and becomes intensely up-regulated in thymus epithelium at day 18 of gestation when TCR β expressing cells have selectively expanded and express both CD4 and CD8. The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR β gene rearrangement. Furthermore, GKLF expression in hematopoietic stroma might suggest the thus far uncharacterised participation of this factor in hematopoiesis.
KW - Gene regulation
KW - Hematopoiesis
KW - Stromal cells
KW - Thymus
KW - Transcription factors
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U2 - 10.1016/S0925-4773(98)00237-8
DO - 10.1016/S0925-4773(98)00237-8
M3 - Article
VL - 81
SP - 103
EP - 113
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -