GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk

Maddalena Panigada, Simona Porcellini, Francesca Sutti, Luisa Doneda, Ombretta Pozzoli, G. Giacomo Consalez, Maria Guttinger, Fabio Grassi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Gut-enriched Kruppel-like factor (GKLF) is a transcriptional regulator expressed in differentiated epithelia. We identified GKLF transcript as a regulated element in thymic epithelium of recombinase-deficient mice during thymus development induced by anti-CD3 antibody injection. This treatment recapitulates the organogenetic process depending on productive rearrangement of T cell receptor (TCR) β gene with thymocytes expansion and acquisition of the CD4+8+ double positive phenotype. In wildtype mice, GKLF is expressed very early in embryogenesis and becomes intensely up-regulated in thymus epithelium at day 18 of gestation when TCR β expressing cells have selectively expanded and express both CD4 and CD8. The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR β gene rearrangement. Furthermore, GKLF expression in hematopoietic stroma might suggest the thus far uncharacterised participation of this factor in hematopoiesis.

Original languageEnglish
Pages (from-to)103-113
Number of pages11
JournalMechanisms of Development
Volume81
Issue number1-2
DOIs
Publication statusPublished - Mar 1 1999

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Thymocytes
Thymus Gland
Epithelium
T-Cell Receptor Genes
T-Lymphocyte Gene Rearrangement
Recombinases
Hematopoiesis
T-Cell Antigen Receptor
Embryonic Development
Anti-Idiotypic Antibodies
GKLF protein
Phenotype
Pregnancy
Injections

Keywords

  • Gene regulation
  • Hematopoiesis
  • Stromal cells
  • Thymus
  • Transcription factors

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk. / Panigada, Maddalena; Porcellini, Simona; Sutti, Francesca; Doneda, Luisa; Pozzoli, Ombretta; Consalez, G. Giacomo; Guttinger, Maria; Grassi, Fabio.

In: Mechanisms of Development, Vol. 81, No. 1-2, 01.03.1999, p. 103-113.

Research output: Contribution to journalArticle

Panigada, M, Porcellini, S, Sutti, F, Doneda, L, Pozzoli, O, Consalez, GG, Guttinger, M & Grassi, F 1999, 'GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk', Mechanisms of Development, vol. 81, no. 1-2, pp. 103-113. https://doi.org/10.1016/S0925-4773(98)00237-8
Panigada, Maddalena ; Porcellini, Simona ; Sutti, Francesca ; Doneda, Luisa ; Pozzoli, Ombretta ; Consalez, G. Giacomo ; Guttinger, Maria ; Grassi, Fabio. / GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk. In: Mechanisms of Development. 1999 ; Vol. 81, No. 1-2. pp. 103-113.
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