GLASS: assisted and standardized assessment of gene variations from Sanger sequence trace data

K Pal, V Bystry, T Reigl, M Demko, A Krejci, T Touloumenidou, E Stalika, B Tichy, P Ghia, K Stamatopoulos, S Pospisilova, J Malcikova, N Darzentas, also on behalf of the European Research Initiative on CLL (ERIC)-TP53 Network

Research output: Contribution to journalArticlepeer-review

Abstract

Motivation: Sanger sequencing is still being employed for sequence variant detection by many laboratories, especially in a clinical setting. However, chromatogram interpretation often requires manual inspection and in some cases, considerable expertise. Results: We present GLASS, a web-based Sanger sequence trace viewer, editor, aligner and variant caller, built to assist with the assessment of variations in 'curated' or user-provided genes. Critically, it produces a standardized variant output as recommended by the Human Genome Variation Society. Availability and implementation: GLASS is freely available at http://bat.infspire.org/genomepd/glass/ with source code at https://github.com/infspiredBAT/GLASS. Contact: nikos.darzentas@gmail.com or malcikova.jitka@fnbrno.cz. Supplementary information: Supplementary data are available at Bioinformatics online.
Original languageEnglish
Pages (from-to)3802-3804
Number of pages3
JournalBioinformatics
Volume33
Issue number23
DOIs
Publication statusPublished - 2017

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