Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization

Giuseppe Terrazzano, Michela Sica, Carmen Gianfrani, Giuseppe Mazzarella, Francesco Maurano, Beatrice De Giulio, Sophie De Saint-Mezard, Delia Zanzi, Luigi Maiuri, Marco Londei, Bana Jabri, Riccardo Troncone, Salvatore Auricchio, Serafino Zappacosta, Ennio Carbone

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We analyzed the autologous NK cell interaction with gliadin-presenting dendritic cells. Gliadin is the known Ag priming the celiac disease (CD) pathogenesis. We demonstrate that gliadin prevents immature dendritic cells (iDCs) elimination by NK cells. Furthermore, cooperation between human NK cells-iDCs and T cells increases IFN-γ production of anti-gliadin immune response. Gliadin fractions were analyzed for their capability to stabilize HLA-E molecules. The α and ω fractions conferred the protection from NK cell lysis to iDCs and increased their HLA-E expression. Gliadin pancreatic enzyme digest and a peptide derived from gliadin a increased HLA-E levels on murine RMA-S/HLA-E-transfected cells. Analysis of HLA-E expression in the small intestinal mucosa of gluten-containing diet celiac patients and organ culture experiments confirmed the in vitro data.

Original languageEnglish
Pages (from-to)372-381
Number of pages10
JournalJournal of Immunology
Volume179
Issue number1
Publication statusPublished - Jul 1 2007

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Gliadin
Natural Killer Cells
Dendritic Cells
Glutens
Organ Culture Techniques
Celiac Disease
Intestinal Mucosa
Cell Communication
Abdomen
Diet
T-Lymphocytes
Peptides
Enzymes

ASJC Scopus subject areas

  • Immunology

Cite this

Terrazzano, G., Sica, M., Gianfrani, C., Mazzarella, G., Maurano, F., De Giulio, B., ... Carbone, E. (2007). Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization. Journal of Immunology, 179(1), 372-381.

Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization. / Terrazzano, Giuseppe; Sica, Michela; Gianfrani, Carmen; Mazzarella, Giuseppe; Maurano, Francesco; De Giulio, Beatrice; De Saint-Mezard, Sophie; Zanzi, Delia; Maiuri, Luigi; Londei, Marco; Jabri, Bana; Troncone, Riccardo; Auricchio, Salvatore; Zappacosta, Serafino; Carbone, Ennio.

In: Journal of Immunology, Vol. 179, No. 1, 01.07.2007, p. 372-381.

Research output: Contribution to journalArticle

Terrazzano, G, Sica, M, Gianfrani, C, Mazzarella, G, Maurano, F, De Giulio, B, De Saint-Mezard, S, Zanzi, D, Maiuri, L, Londei, M, Jabri, B, Troncone, R, Auricchio, S, Zappacosta, S & Carbone, E 2007, 'Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization', Journal of Immunology, vol. 179, no. 1, pp. 372-381.
Terrazzano G, Sica M, Gianfrani C, Mazzarella G, Maurano F, De Giulio B et al. Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization. Journal of Immunology. 2007 Jul 1;179(1):372-381.
Terrazzano, Giuseppe ; Sica, Michela ; Gianfrani, Carmen ; Mazzarella, Giuseppe ; Maurano, Francesco ; De Giulio, Beatrice ; De Saint-Mezard, Sophie ; Zanzi, Delia ; Maiuri, Luigi ; Londei, Marco ; Jabri, Bana ; Troncone, Riccardo ; Auricchio, Salvatore ; Zappacosta, Serafino ; Carbone, Ennio. / Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization. In: Journal of Immunology. 2007 ; Vol. 179, No. 1. pp. 372-381.
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