Glial and neuronal cells express functional chemokine receptor CXCR4 and its natural ligand stromal cell-derived factor 1

Adriana Bajetto, Rudy Bonavia, Simone Barbero, Patrizia Piccioli, Alfredo Costa, Tullio Florio, Gennaro Schettini

Research output: Contribution to journalArticlepeer-review

Abstract

Chemokines are a family of proteins that chemoattract and activate cells by interacting with specific receptors on the surface of their targets. The chemokine stromal cell-derived factor 1, (SDF1), binds to the seventransmembrane G protein-coupled CXCR4 receptor and acts to modulate cell migration, differentiation, and proliferation. CXCR4 and SDF1 are reported to be expressed in various tissues including brain. Here we show that SDF1 and CXCR4 are expressed in cultured cortical type I rat astrocytes, cortical neurons, and cerebellar granule cells. In cortical astrocytes, prolonged treatment with lipopolysaccharide induced an increase of SDF1 expression and a down-regulation of CXCR4, whereas treatment with phorbol esters did not affect SDF1 expression and down-modulated CXCR4 receptor expression. We also demonstrated the ability of human SDF1 α (hSDF1α) to increase the intracellular calcium level in cultured astrocytes and cortical neurons, whereas in the same conditions, cerebellar granule cells did not modify their intracellular calcium concentration. Furthermore, in cortical astrocytes, the simultaneous treatment of hSDF1α with the HIV-1 capside glycoprotein gp120 inhibits the cyclic AMP formation induced by forskolin treatment.

Original languageEnglish
Pages (from-to)2348-2357
Number of pages10
JournalJournal of Neurochemistry
Volume73
Issue number6
DOIs
Publication statusPublished - 1999

Keywords

  • Astrocytes
  • Cerebellar granule cells
  • Cortical neurons
  • CXCR4 chemokine receptor
  • Stromal cell-derived factor 1

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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