Glioblastoma stem cells: Radiobiological response to ionising radiations of different qualities

I. Pecchia, V. Dini, L. Ricci-Vitiani, M. Biffoni, M. Balduzzi, E. Fratini, M. Belli, A. Campa, G. Esposito, G. Cirrone, F. Romano, C. Stancampiano, F. Pelacchi, R. Pallini, M. A. Tabocchini

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumour, with very poor prognosis. The high recurrence rate and failure of conventional treatments are expected to be related to the presence of radio-resistant cancer stem cells (CSCs) inside the tumour mass. CSCs can both self-renew and differentiate into the heterogeneous lineages of cancer cells. Recent evidence showed a higher effectiveness of C-ions and protons in inactivating CSCs, suggesting a potential advantage of Hadrontherapy compared with conventional radiotherapy for GBM treatment. To investigate the mechanisms involved in the molecular and cellular responses of CSCs to ionising radiations, two GBM stem cell (GSC) lines, named lines 1 and 83, which were derived from patients with different clinical outcomes and having different metabolic profiles (as shown by NMR spectroscopy), were irradiated with 137Cs photons and with protons or C-ions of 62 MeV u-1 in the dose range of 5-40 Gy. The biological effects investigated were: cell death, cell cycle progression, and DNA damage induction and repair. Preliminary results show a different response to ionising radiation between the two GSC lines for the different end points investigated. Further experiments are in progress to consolidate the data and to get more insights on the influence of radiation quality.

Original languageEnglish
Article numberncv299
Pages (from-to)374-378
Number of pages5
JournalRadiation Protection Dosimetry
Volume166
Issue number1-4
DOIs
Publication statusPublished - Sep 1 2015

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging
  • Public Health, Environmental and Occupational Health

Fingerprint Dive into the research topics of 'Glioblastoma stem cells: Radiobiological response to ionising radiations of different qualities'. Together they form a unique fingerprint.

  • Cite this