Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals

Sofia Pavanello, Valentina Bollati, Angela Cecilia Pesatori, Lucyna Kapka, Claudia Bolognesi, Pier Alberto Bertazzi, Andrea Baccarelli

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122 Citations (Scopus)

Abstract

We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.

Original languageEnglish
Pages (from-to)1692-1697
Number of pages6
JournalInternational Journal of Cancer
Volume125
Issue number7
DOIs
Publication statusPublished - Oct 1 2009

Fingerprint

DNA Adducts
Polycyclic Aromatic Hydrocarbons
DNA Methylation
Methylation
Long Interspersed Nucleotide Elements
Coke
Lymphocytes
Genes
Chromosomal Instability
Benzo(a)pyrene
Cytosine
Interleukin-6

Keywords

  • anti-B[a]PDE-DNA adduct
  • Chronic exposure
  • DNA methylation
  • Lung carcinogens
  • Micronuclei
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals",
abstract = "We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines ({\%}mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.",
keywords = "anti-B[a]PDE-DNA adduct, Chronic exposure, DNA methylation, Lung carcinogens, Micronuclei, Polycyclic aromatic hydrocarbons",
author = "Sofia Pavanello and Valentina Bollati and Pesatori, {Angela Cecilia} and Lucyna Kapka and Claudia Bolognesi and Bertazzi, {Pier Alberto} and Andrea Baccarelli",
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AU - Pavanello, Sofia

AU - Bollati, Valentina

AU - Pesatori, Angela Cecilia

AU - Kapka, Lucyna

AU - Bolognesi, Claudia

AU - Bertazzi, Pier Alberto

AU - Baccarelli, Andrea

PY - 2009/10/1

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N2 - We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.

AB - We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.

KW - anti-B[a]PDE-DNA adduct

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KW - DNA methylation

KW - Lung carcinogens

KW - Micronuclei

KW - Polycyclic aromatic hydrocarbons

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