Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals

Sofia Pavanello, Valentina Bollati, Angela Cecilia Pesatori, Lucyna Kapka, Claudia Bolognesi, Pier Alberto Bertazzi, Andrea Baccarelli

Research output: Contribution to journalArticle

Abstract

We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke-oven workers and matched controls. Methylation states of gene-specific promoters (p53, p16, HIC1 and IL-6) and of Alu and LINE-1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1-pyrenol and anti-benzo[a]pyrene diolepoxide (anti-B[a]PDE)-DNA adduct levels, a critical genetic damage from B[a]P. We also evaluated whether PAH-induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability.

Original languageEnglish
Pages (from-to)1692-1697
Number of pages6
JournalInternational Journal of Cancer
Volume125
Issue number7
DOIs
Publication statusPublished - Oct 1 2009

Keywords

  • anti-B[a]PDE-DNA adduct
  • Chronic exposure
  • DNA methylation
  • Lung carcinogens
  • Micronuclei
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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