Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos

Alessia Gallo; Serena Vella; Monica Miele; Francesca Timoneri; Mariangela Di Bella; Silvia Bosi; Marco Sciveres; Pier Giulio Conaldi

doi:10.1016/j.canlet.2016.12.003

Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos

Alessia Gallo, Serena Vella, Monica Miele, Francesca Timoneri, Mariangela Di Bella, Silvia Bosi, Marco Sciveres, Pier Giulio Conaldi

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Abstract

The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein-Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors. In this study, we have demonstrated, for the first time, that all the viral miRNAs expressed by LCL are also packaged in the exosomes, and we found that two miRNAs, ebv-miR-BART3 and ebv-miR-BHRF1-1, are more abundant in the exosomes, suggesting a microvescicular viral microRNA transfer. In addition, lncRNA profiling revealed that LCLs were enriched in lncRNA H19 and H19 antisense, and released these through exosomes, suggesting a leading role in the regulation of the tumor microenvironment.

Original language	English
Pages (from-to)	334-343
Number of pages	10
Journal	Cancer Letters
Volume	388
DOIs	https://doi.org/10.1016/j.canlet.2016.12.003
Publication status	Published - Mar 1 2017

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Keywords

Cell Line, Tumor
Epstein-Barr Virus Infections
Exosomes
Humans
Lymphoma
RNA, Long Noncoding
Journal Article

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Gallo, A., Vella, S., Miele, M., Timoneri, F., Di Bella, M., Bosi, S., Sciveres, M., & Conaldi, P. G. (2017). Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos. Cancer Letters, 388, 334-343. https://doi.org/10.1016/j.canlet.2016.12.003

Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos. / Gallo, Alessia; Vella, Serena; Miele, Monica; Timoneri, Francesca; Di Bella, Mariangela; Bosi, Silvia; Sciveres, Marco ; Conaldi, Pier Giulio.

In: Cancer Letters, Vol. 388, 01.03.2017, p. 334-343.

Research output: Contribution to journal › Article

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abstract = "The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein-Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors. In this study, we have demonstrated, for the first time, that all the viral miRNAs expressed by LCL are also packaged in the exosomes, and we found that two miRNAs, ebv-miR-BART3 and ebv-miR-BHRF1-1, are more abundant in the exosomes, suggesting a microvescicular viral microRNA transfer. In addition, lncRNA profiling revealed that LCLs were enriched in lncRNA H19 and H19 antisense, and released these through exosomes, suggesting a leading role in the regulation of the tumor microenvironment.",

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AU - Timoneri, Francesca

AU - Di Bella, Mariangela

AU - Bosi, Silvia

AU - Sciveres, Marco

AU - Conaldi, Pier Giulio

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AB - The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein-Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors. In this study, we have demonstrated, for the first time, that all the viral miRNAs expressed by LCL are also packaged in the exosomes, and we found that two miRNAs, ebv-miR-BART3 and ebv-miR-BHRF1-1, are more abundant in the exosomes, suggesting a microvescicular viral microRNA transfer. In addition, lncRNA profiling revealed that LCLs were enriched in lncRNA H19 and H19 antisense, and released these through exosomes, suggesting a leading role in the regulation of the tumor microenvironment.

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10.1016/j.canlet.2016.12.003