Glucocorticoid treatment reduces T-bet and pSTAT1 expression in mononuclear cells from relapsing remitting multiple sclerosis patients

Giovanni Frisullo, Viviana Nociti, Raffaele Iorio, Agata Katia Patanella, Assunta Bianco, Marcella Caggiula, Cristina Sancricca, Pietro Attilio Tonali, Massimiliano Mirabella, Anna Paola Batocchi

Research output: Contribution to journalArticle

Abstract

High dose glucocorticoid (GC) treatment has been demonstrated to have a short-term beneficial effect on functional recovery in relapsing multiple sclerosis (MS) patients but the exact mechanism of action of GCs in MS is unclear. We found that high dose intravenous GCs strongly reduced T-bet and pSTAT1 expression in CD4+, CD8+, CD14+ circulating cells in RRMS patients in relapse. pSTAT1and T-bet reduction was associated with the decline of IFNγ production by PBMCs. A significant increase of AV-positive CD4+ and CD8+ T cells was detectable after GC treatment without any variation in the percentage of annexin V-positive monocytes. By in vitro analysis, patients during relapse, either before or after GC treatment, exhibited a lower proportion of apoptotic lymphocytes than remitting patients and controls. Our study suggests that GCs can modulate T-bet and STAT1 expression and that IFNγ signalling inhibition contributes to anti-inflammatory action of GCs in the treatment of relapses of MS patients.

Original languageEnglish
Pages (from-to)284-293
Number of pages10
JournalClinical Immunology
Volume124
Issue number3
DOIs
Publication statusPublished - Sep 2007

Keywords

  • Glucocorticoid
  • Multiple sclerosis
  • STAT-1
  • T-bet

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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    Frisullo, G., Nociti, V., Iorio, R., Katia Patanella, A., Bianco, A., Caggiula, M., Sancricca, C., Tonali, P. A., Mirabella, M., & Batocchi, A. P. (2007). Glucocorticoid treatment reduces T-bet and pSTAT1 expression in mononuclear cells from relapsing remitting multiple sclerosis patients. Clinical Immunology, 124(3), 284-293. https://doi.org/10.1016/j.clim.2007.05.011