TY - JOUR
T1 - Glucose availability enhances lipopolysaccharide production and immunogenicity in the opportunistic pathogen Acinetobacter baumannii
AU - Rossi, Elio
AU - Longo, Francesca
AU - Barbagallo, Marialuisa
AU - Peano, Clelia
AU - Consolandi, Clarissa
AU - Pietrelli, Alessandro
AU - Jaillon, Sebastian
AU - Garlanda, Cecilia
AU - Landini, Paolo
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Aim: Acinetobacter baumannii can cause sepsis with high mortality rates. We investigated whether glucose sensing might play a role in A. baumannii pathogenesis. Materials & methods: We carried out transcriptome analysis and extracellular polysaccharide determination in an A. baumannii clinical isolate grown on complex medium with or without glucose supplementation, and assessed its ability to induce production of inflammatory cytokines in human macrophages. Results: Growth in glucose-supplemented medium strongly enhanced A. baumannii sugar anabolism, resulting in increasing lipopolysaccharide biosynthesis. In addition, glucose induced active shedding of lipopolysaccharide, in turn triggering a strong induction of inflammatory cytokines in human macrophages. Finally, hemolytic activity was strongly enhanced by growth in glucose-supplemented medium. Conclusion: We propose that sensing of exogenous glucose might trigger A. baumannii pathogenesis during sepsis.
AB - Aim: Acinetobacter baumannii can cause sepsis with high mortality rates. We investigated whether glucose sensing might play a role in A. baumannii pathogenesis. Materials & methods: We carried out transcriptome analysis and extracellular polysaccharide determination in an A. baumannii clinical isolate grown on complex medium with or without glucose supplementation, and assessed its ability to induce production of inflammatory cytokines in human macrophages. Results: Growth in glucose-supplemented medium strongly enhanced A. baumannii sugar anabolism, resulting in increasing lipopolysaccharide biosynthesis. In addition, glucose induced active shedding of lipopolysaccharide, in turn triggering a strong induction of inflammatory cytokines in human macrophages. Finally, hemolytic activity was strongly enhanced by growth in glucose-supplemented medium. Conclusion: We propose that sensing of exogenous glucose might trigger A. baumannii pathogenesis during sepsis.
KW - Acinetobacter baumannii
KW - glucose
KW - host-pathogen interactions
KW - innate immune response
KW - LPS
KW - macrophage
KW - sepsis transcriptomics
KW - virulence factors
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U2 - 10.2217/fmb.15.153
DO - 10.2217/fmb.15.153
M3 - Article
AN - SCOPUS:84962467638
VL - 11
SP - 335
EP - 349
JO - Future Microbiology
JF - Future Microbiology
SN - 1746-0913
IS - 3
ER -