Glucose utilization by tumor cells: A post-translational modification of mitochondrial hexokinase may play a regulatory role

Marco G. Paggi, Maurizio Fanciulli, Carlo Del Carlo, Gennaro Citro, Tiziana Bruno, Aristide Floridi

Research output: Contribution to journalArticle

Abstract

In Northern blot analysis of a series of tumor cell lines a single hexokinase mRNA species of 4.3 Kb was detected. Detailed examination of one such line, the rat AS-30D hepatoma, revealed that two mitochondrial species of hexokinase are present with a molecular mass of 115 and 107 KDa. The smaller of the two species is 4-fold more active than the larger. Only the larger, less active species is detected in the well differentiated H-35 rat hepatoma cell line which exibits a lower glucose catabolic rate. These results suggest that a post-translational proteolytic event may play a central role in regulating the glucose utilization capacity of tumor cells by modulating the relative levels of high and low activity forms of hexokinase.

Original languageEnglish
Pages (from-to)648-655
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume178
Issue number2
DOIs
Publication statusPublished - Jul 31 1991

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Hexokinase
Post Translational Protein Processing
Tumors
Cells
Glucose
Rats
Hepatocellular Carcinoma
Neoplasms
Molecular mass
Tumor Cell Line
Northern Blotting
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Glucose utilization by tumor cells : A post-translational modification of mitochondrial hexokinase may play a regulatory role. / Paggi, Marco G.; Fanciulli, Maurizio; Del Carlo, Carlo; Citro, Gennaro; Bruno, Tiziana; Floridi, Aristide.

In: Biochemical and Biophysical Research Communications, Vol. 178, No. 2, 31.07.1991, p. 648-655.

Research output: Contribution to journalArticle

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