Glucose utilization by tumor cells: A post-translational modification of mitochondrial hexokinase may play a regulatory role

Marco G. Paggi, Maurizio Fanciulli, Carlo Del Carlo, Gennaro Citro, Tiziana Bruno, Aristide Floridi

Research output: Contribution to journalArticlepeer-review

Abstract

In Northern blot analysis of a series of tumor cell lines a single hexokinase mRNA species of 4.3 Kb was detected. Detailed examination of one such line, the rat AS-30D hepatoma, revealed that two mitochondrial species of hexokinase are present with a molecular mass of 115 and 107 KDa. The smaller of the two species is 4-fold more active than the larger. Only the larger, less active species is detected in the well differentiated H-35 rat hepatoma cell line which exibits a lower glucose catabolic rate. These results suggest that a post-translational proteolytic event may play a central role in regulating the glucose utilization capacity of tumor cells by modulating the relative levels of high and low activity forms of hexokinase.

Original languageEnglish
Pages (from-to)648-655
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume178
Issue number2
DOIs
Publication statusPublished - Jul 31 1991

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Glucose utilization by tumor cells: A post-translational modification of mitochondrial hexokinase may play a regulatory role'. Together they form a unique fingerprint.

Cite this