Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones

Santina Bruzzone, Claudia Verderio, Ursula Schenk, Ernesto Fedele, Elena Zocchi, Michela Matteolit, Antonio De Flora

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Recently, a new system of astrocyte-neurone glutamatergic signalling has been identified. It is started in astrocytes by ectocellular, CD38-catalysed conversion of NAD+ to the calcium mobilizer cyclic ADP-ribose (cADPR). This is then pumped by CD38 itself into the cytosol where the resulting free intracellular Ca2+ concentration [Ca 2+]i transients elicit an increased release of glutamate, which can induce an enhanced Ca2+ response in neighbouring neurones. Here, we demonstrate that co-culture of either cortical or hippocampal astrocytes with neurones results in a significant overexpression of astrocyte CD38 both on the plasma membrane and intracellularly. The causal role of neurone-released glutamate in inducing overexpression of astrocyte CD38 is demonstrated by two observations: first, in the absence of neurones, induction of CD38 in pure astrocyte cultures can be obtained with glutamate and second, it can be prevented in co-cultures by glutamate receptor antagonists. The neuronal glutamate-mediated effect of neurones on astrocyte CD38 expression is paralleled by increased intracellular cADPR and [Ca2+]i levels, both findings indicating functionality of overexpressed CD38. These results reveal a new neurone-to-astrocyte glutamatergic signalling based on the CD38/cADPR system, which affects the [Ca2+]i in both cell types, adding further complexity to the bi-directional patterns of communication between astrocytes and neurones.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalJournal of Neurochemistry
Volume89
Issue number1
Publication statusPublished - Apr 2004

Fingerprint

Astrocytes
Neurons
Glutamic Acid
Cyclic ADP-Ribose
Coculture Techniques
Excitatory Amino Acid Antagonists
Cell membranes
NAD
Cytosol
Cell Membrane
Calcium
Communication

Keywords

  • Astrocytes
  • CD38
  • Cyclic ADP-ribose
  • Glutamate
  • Intracellular calcium
  • Neurones

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Bruzzone, S., Verderio, C., Schenk, U., Fedele, E., Zocchi, E., Matteolit, M., & De Flora, A. (2004). Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. Journal of Neurochemistry, 89(1), 264-272.

Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. / Bruzzone, Santina; Verderio, Claudia; Schenk, Ursula; Fedele, Ernesto; Zocchi, Elena; Matteolit, Michela; De Flora, Antonio.

In: Journal of Neurochemistry, Vol. 89, No. 1, 04.2004, p. 264-272.

Research output: Contribution to journalArticle

Bruzzone, S, Verderio, C, Schenk, U, Fedele, E, Zocchi, E, Matteolit, M & De Flora, A 2004, 'Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones', Journal of Neurochemistry, vol. 89, no. 1, pp. 264-272.
Bruzzone S, Verderio C, Schenk U, Fedele E, Zocchi E, Matteolit M et al. Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. Journal of Neurochemistry. 2004 Apr;89(1):264-272.
Bruzzone, Santina ; Verderio, Claudia ; Schenk, Ursula ; Fedele, Ernesto ; Zocchi, Elena ; Matteolit, Michela ; De Flora, Antonio. / Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. In: Journal of Neurochemistry. 2004 ; Vol. 89, No. 1. pp. 264-272.
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