Glutamate release in the nucleus tractus solitarius induced by peripheral lipopolysaccharide and interleukin-1β

P. Mascarucci, C. Perego, S. Terrazzino, M. G. De Simoni

Research output: Contribution to journalArticle

Abstract

The involvement of vagal afferents in the communication pathway from the immune system to the brain was studied. Glutamate was measured in the nucleus tractus solitarius, the brain area receiving glutamatergic vagal afferents, after peripheral injection of lipopolysaccharide or interleukin-1β. Intraperitoneal or intravenous saline or intraperitoneal heat-inactivated interleukin-1β increased glutamate release, measured by brain microdialysis in freely-moving rats at 20 min (137±19%, 126±10% and 133±6%, respectively), without inducing any other change up to 3 h after injection. Intraperitoneal lipopolysaccharide (10 μg/rat) increased glutamate at 20 min (132 ± 10%) and at 60 min (208 ± 26%). To compare lipopolysaccharide effectiveness by the two routes, serum levels of interleukin-1β, interleukin-6 and tumour necrosis factor-α were measured. Intravenous lipopolysaccharide induced each cytokine more rapidly and efficiently than intraperitoneal lipopolysaccharide. Perfusion with tetrodotoxin (1 μM) in the dialysis probe decreased glutamate basal levels by approximately 20% and completely prevented lipopolysaccharide effects, showing the neuronal component of the glutamate measured. Except for the 20-min increase, intravenous lipopolysaccharide (10 μg/rat) did not affect glutamate release. Intraperitoneal interleukin- 1β (4 μg/rat) increased glutamate release at 20 min (126 ± 6%) and at 40 min (150 ± 18%). These data indicate that vagal glutamatergic system in the nucleus tractus solitarius is activated by intraperitoneal injections of immunoactive compounds.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
JournalNeuroscience
Volume86
Issue number4
DOIs
Publication statusPublished - Jun 18 1998

Fingerprint

Solitary Nucleus
Interleukin-1
Lipopolysaccharides
Glutamic Acid
Brain
Injections
Microdialysis
Tetrodotoxin
Intraperitoneal Injections
Interleukin-4
Dialysis
Immune System
Interleukin-6
Tumor Necrosis Factor-alpha
Perfusion
Hot Temperature
Communication
Cytokines
Serum

Keywords

  • Brain microdialysis
  • Glutamate
  • Interleukin-1β
  • Lipopolysaccharide
  • Nucleus tractus solitarius
  • Vagal afferents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Glutamate release in the nucleus tractus solitarius induced by peripheral lipopolysaccharide and interleukin-1β. / Mascarucci, P.; Perego, C.; Terrazzino, S.; De Simoni, M. G.

In: Neuroscience, Vol. 86, No. 4, 18.06.1998, p. 1285-1290.

Research output: Contribution to journalArticle

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