Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients

L. Tremolizzo, G. Sala, L. Stanzani, A. Aliprandi, B. Begni, C. Zoia, L. Brighina, E. Beghi, L. Frattola, C. Ferrarese

Research output: Contribution to journalArticle

Abstract

Excitotoxicity seems to play a major role in motor neuron degeneration in amyotrophic lateral sclerosis (ALS). Decreased glutamate uptake has been shown in the synaptosomes from spinal cord and motor cortex of ALS patients. The uptake decrease is associated with altered expression of the astrocytic glutamate transporter EAAT2 (GLT-1), which has been linked to abnormal mRNA splicing or to protein modification induced by oxidative damage. To assess whether glutamate uptake can be a marker of disease progression and/or is observed in genetically susceptible individuals, we used platelets, which possess a high-affinity glutamate uptake similar to that described in brain synaptosomes, and all major glutamate transporters. Glutamate uptake was determined in platelets from 56 sporadic ALS (SALS) patients, 29 relatives, and 47 age-related normal controls, as Na+-dependent [3H]glutamate influx. EAAT2 expression was investigated by Western blot analysis using polyclonal rabbit antibodies. A 31% reduction of high-affinity glutamate uptake rate (P

Original languageEnglish
JournalNeurological Sciences
Volume21
Issue number4 SUPPL.
Publication statusPublished - 2000

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Glutamic Acid
Blood Platelets
Amino Acid Transport System X-AG
Synaptosomes
Amyotrophic Lateral Sclerosis
Nerve Degeneration
Motor Cortex
Motor Neurons
Disease Progression
Amyotrophic lateral sclerosis 1
Spinal Cord
Western Blotting
Rabbits
Messenger RNA
Antibodies
Brain
Proteins

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Tremolizzo, L., Sala, G., Stanzani, L., Aliprandi, A., Begni, B., Zoia, C., ... Ferrarese, C. (2000). Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients. Neurological Sciences, 21(4 SUPPL.).

Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients. / Tremolizzo, L.; Sala, G.; Stanzani, L.; Aliprandi, A.; Begni, B.; Zoia, C.; Brighina, L.; Beghi, E.; Frattola, L.; Ferrarese, C.

In: Neurological Sciences, Vol. 21, No. 4 SUPPL., 2000.

Research output: Contribution to journalArticle

Tremolizzo, L, Sala, G, Stanzani, L, Aliprandi, A, Begni, B, Zoia, C, Brighina, L, Beghi, E, Frattola, L & Ferrarese, C 2000, 'Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients', Neurological Sciences, vol. 21, no. 4 SUPPL..
Tremolizzo L, Sala G, Stanzani L, Aliprandi A, Begni B, Zoia C et al. Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients. Neurological Sciences. 2000;21(4 SUPPL.).
Tremolizzo, L. ; Sala, G. ; Stanzani, L. ; Aliprandi, A. ; Begni, B. ; Zoia, C. ; Brighina, L. ; Beghi, E. ; Frattola, L. ; Ferrarese, C. / Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients. In: Neurological Sciences. 2000 ; Vol. 21, No. 4 SUPPL.
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