Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients

L. Tremolizzo, G. Sala, L. Stanzani, A. Aliprandi, B. Begni, C. Zoia, L. Brighina, E. Beghi, L. Frattola, C. Ferrarese

Research output: Contribution to journalArticle

Abstract

Excitotoxicity seems to play a major role in motor neuron degeneration in amyotrophic lateral sclerosis (ALS). Decreased glutamate uptake has been shown in the synaptosomes from spinal cord and motor cortex of ALS patients. The uptake decrease is associated with altered expression of the astrocytic glutamate transporter EAAT2 (GLT-1), which has been linked to abnormal mRNA splicing or to protein modification induced by oxidative damage. To assess whether glutamate uptake can be a marker of disease progression and/or is observed in genetically susceptible individuals, we used platelets, which possess a high-affinity glutamate uptake similar to that described in brain synaptosomes, and all major glutamate transporters. Glutamate uptake was determined in platelets from 56 sporadic ALS (SALS) patients, 29 relatives, and 47 age-related normal controls, as Na+-dependent [3H]glutamate influx. EAAT2 expression was investigated by Western blot analysis using polyclonal rabbit antibodies. A 31% reduction of high-affinity glutamate uptake rate (P

Original languageEnglish
JournalNeurological Sciences
Volume21
Issue number4 SUPPL.
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients'. Together they form a unique fingerprint.

  • Cite this

    Tremolizzo, L., Sala, G., Stanzani, L., Aliprandi, A., Begni, B., Zoia, C., Brighina, L., Beghi, E., Frattola, L., & Ferrarese, C. (2000). Glutamate uptake and GLT-1 expression in platelets from sporadic amyotrophic lateral sclerosis patients. Neurological Sciences, 21(4 SUPPL.).