Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes

Michele Ettorre, Erika Lorenzetto, Claudia Laperchia, Cristina Baiguera, Caterina Branca, Manuela Benarese, PierFranco Spano, Marina Pizzi, Mario Buffelli

Research output: Contribution to journalArticle

Abstract

Background: The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known. Methods and Findings: To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction. Conclusions: These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures.

Original languageEnglish
Article numbere31451
JournalPLoS One
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 9 2012

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Skeletal Muscle Fibers
synapse
Synapses
myocytes
Muscle Cells
fluorescent antibody technique
Neurons
Fluorescent Antibody Technique
Muscle
neurons
Cells
Electrophysiology
electrophysiology
cholinergic receptors
Neuromuscular Junction
Neurology
Cholinergic Receptors
Immunoprecipitation
nervous system
Nervous System

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ettorre, M., Lorenzetto, E., Laperchia, C., Baiguera, C., Branca, C., Benarese, M., ... Buffelli, M. (2012). Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes. PLoS One, 7(2), [e31451]. https://doi.org/10.1371/journal.pone.0031451

Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes. / Ettorre, Michele; Lorenzetto, Erika; Laperchia, Claudia; Baiguera, Cristina; Branca, Caterina; Benarese, Manuela; Spano, PierFranco; Pizzi, Marina; Buffelli, Mario.

In: PLoS One, Vol. 7, No. 2, e31451, 09.02.2012.

Research output: Contribution to journalArticle

Ettorre, M, Lorenzetto, E, Laperchia, C, Baiguera, C, Branca, C, Benarese, M, Spano, P, Pizzi, M & Buffelli, M 2012, 'Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes', PLoS One, vol. 7, no. 2, e31451. https://doi.org/10.1371/journal.pone.0031451
Ettorre M, Lorenzetto E, Laperchia C, Baiguera C, Branca C, Benarese M et al. Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes. PLoS One. 2012 Feb 9;7(2). e31451. https://doi.org/10.1371/journal.pone.0031451
Ettorre, Michele ; Lorenzetto, Erika ; Laperchia, Claudia ; Baiguera, Cristina ; Branca, Caterina ; Benarese, Manuela ; Spano, PierFranco ; Pizzi, Marina ; Buffelli, Mario. / Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes. In: PLoS One. 2012 ; Vol. 7, No. 2.
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