Glutamine synthetase desensitizes differentiated adipocytes to proinflammatory stimuli by raising intracellular glutamine levels

Erika Mariana Palmieri, Iolanda Spera, Alessio Menga, Vittoria Infantino, Vito Iacobazzi, Alessandra Castegna

Research output: Contribution to journalArticlepeer-review

Abstract

The role of glutamine synthetase (GS) during adipocyte differentiation is unclear. Here, we assess the impact of GS on the adipocytic response to a proinflammatory challenge at different differentiation stages. GS expression at the late stages of differentiation desensitized mature adipocytes to bacterial lipopolysaccharide (LPS) by increasing intracellular glutamine levels. Furthermore, LPS-activated mature adipocytes were unable to produce inflammatory mediators; LPS sensitivity was rescued following GS inhibition and the associated drop in intracellular glutamine levels. The ability of adipocytes to differentially respond to LPS during differentiation negatively correlates to GS expression and intracellular glutamine levels. Hence, modulation of intracellular glutamine levels by GS expression represents an endogenous mechanism through which mature adipocytes control the inflammatory response.

Original languageEnglish
Pages (from-to)4807-4814
Number of pages8
JournalFEBS Letters
Volume588
Issue number24
DOIs
Publication statusPublished - Dec 20 2014

Keywords

  • Adipocyte
  • Glutamine
  • Glutamine synthetase
  • Inflammation
  • Metabolic syndrome

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

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