Glutathione levels and sensitivity to apoptosis are regulated by changes in transaldolase expression

Katalin Banki, Eliza Hutter, Emanuela Colombo, Nick J. Gonchoroff, Andras Perl

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Transaldolase (TAL) is a key enzyme of the reversible nonoxidative branch of the pentose phosphate pathway (PPP) that is responsible for the generation of NADPH to maintain glutathione at a reduced state (GSH) and, thus, to protect cellular integrity from reactive oxygen intermediates (ROIs). Formation of ROIs have been implicated in certain types of apoptotic cell death. To evaluate the role of TAL in this process, Jurkat human T cells were permanently transfected with TAL expression vectors oriented in the sense or antisense direction. Overexpression of TAL resulted in a decrease in glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities and NADFH and GSH levels and rendered these cells highly susceptible to apoptosis induced by serum deprivation, hydrogen peroxide, nitric oxide, tumor necrosis factor-α, and anti-Fas monoclonal antibody. In addition, reduced levels of TAL resulted in increased glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities and increased GSH levels with inhibition of apoptosis in all five model systems. The effect of TAL expression on susceptibility to apoptosis through regulating the PPP and GSH production is consistent with an involvement of ROIs in each pathway tested. Production of ROIs in Fas-mediated cell death was further substantiated by measurement of intracellular ROI production with oxidation- sensitive fluorescent probes, by the protective effects of GSH precursor, N- acetyl cysteine, free radical spin traps 5,5-dimethyl-1-pyrroline-1-oxide and 3,3,5,5-tetramethyl-1-pyrroline-1-oxide, the antioxidants desferrioxamine, nordihydroguaiaretic acid, and Amytal, and by the enhancing effects of GSH depletion with buthionine sulfoximine. The results provide definitive evidence that TAL has a role in regulating the balance between the two branches of PPP and its overall output as measured by GSH production and thus influences sensitivity to cell death signals.

Original languageEnglish
Pages (from-to)32994-33001
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number51
DOIs
Publication statusPublished - 1996

Fingerprint

Transaldolase
Glutathione
Apoptosis
Pentoses
Pentose Phosphate Pathway
Oxygen
Cell death
Phosphogluconate Dehydrogenase
Cell Death
Glucosephosphate Dehydrogenase
anti-Fas monoclonal antibody
Phosphates
Acetylcysteine
Masoprocol
Amobarbital
Buthionine Sulfoximine
Deferoxamine
T-cells
NADP
Fluorescent Dyes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glutathione levels and sensitivity to apoptosis are regulated by changes in transaldolase expression. / Banki, Katalin; Hutter, Eliza; Colombo, Emanuela; Gonchoroff, Nick J.; Perl, Andras.

In: Journal of Biological Chemistry, Vol. 271, No. 51, 1996, p. 32994-33001.

Research output: Contribution to journalArticle

Banki, Katalin ; Hutter, Eliza ; Colombo, Emanuela ; Gonchoroff, Nick J. ; Perl, Andras. / Glutathione levels and sensitivity to apoptosis are regulated by changes in transaldolase expression. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 51. pp. 32994-33001.
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