Glutathione pretreatment lessens the acute liver injury induced by carbon tetrachloride

Beatrice Arosio, Daniela Santambrogio, Nicoletta Gagliano, Anthony Ryan, Fiorella Biasi, Carlo Vergani, Giorgio Annoni

Research output: Contribution to journalArticle

Abstract

We explored the protective activity of glutathione (GSH) in a rat model of carbon tetrachloride-induced acute liver damage. Histological examination of livers from GSH-pretreated rats revealed minor damage, confirmed by biochemical parameters of liver cell necrosis evaluated both 24 and 48 hr after hepatotoxin delivery. In addition we quantified changes in hepatic steady-state levels of albumin, type I procollagen, transforming growth factor-β1 and tumour necrosis factor-α mRNAs. Even at the molecular level and above all for the albumin gene, it appears that GSH lessens the effect of the hepatotoxin, however the protection of the thiol is restricted to the first 24 hrs and is almost totally exhausted after 48 hr. Since, only 24 hr after CCl4 delivery, GSH abundance determined in erythrocytes and liver is almost equal in the controls and in the GSH injected rats, but significantly higher than in the only intoxicated animals (P <0.05, intraerythrocyte content), we conclude that the thiol pretreatment exerts an effective but transient protection.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalPharmacology and Toxicology
Volume81
Issue number4
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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    Arosio, B., Santambrogio, D., Gagliano, N., Ryan, A., Biasi, F., Vergani, C., & Annoni, G. (1997). Glutathione pretreatment lessens the acute liver injury induced by carbon tetrachloride. Pharmacology and Toxicology, 81(4), 164-168.