Abstract
We explored the protective activity of glutathione (GSH) in a rat model of carbon tetrachloride-induced acute liver damage. Histological examination of livers from GSH-pretreated rats revealed minor damage, confirmed by biochemical parameters of liver cell necrosis evaluated both 24 and 48 hr after hepatotoxin delivery. In addition we quantified changes in hepatic steady-state levels of albumin, type I procollagen, transforming growth factor-β1 and tumour necrosis factor-α mRNAs. Even at the molecular level and above all for the albumin gene, it appears that GSH lessens the effect of the hepatotoxin, however the protection of the thiol is restricted to the first 24 hrs and is almost totally exhausted after 48 hr. Since, only 24 hr after CCl4 delivery, GSH abundance determined in erythrocytes and liver is almost equal in the controls and in the GSH injected rats, but significantly higher than in the only intoxicated animals (P <0.05, intraerythrocyte content), we conclude that the thiol pretreatment exerts an effective but transient protection.
| Original language | English |
|---|---|
| Pages (from-to) | 164-168 |
| Number of pages | 5 |
| Journal | Pharmacology and Toxicology |
| Volume | 81 |
| Issue number | 4 |
| Publication status | Published - 1997 |
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ASJC Scopus subject areas
- Pharmacology
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Glutathione pretreatment lessens the acute liver injury induced by carbon tetrachloride. / Arosio, Beatrice; Santambrogio, Daniela; Gagliano, Nicoletta; Ryan, Anthony; Biasi, Fiorella; Vergani, Carlo; Annoni, Giorgio.
In: Pharmacology and Toxicology, Vol. 81, No. 4, 1997, p. 164-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glutathione pretreatment lessens the acute liver injury induced by carbon tetrachloride
AU - Arosio, Beatrice
AU - Santambrogio, Daniela
AU - Gagliano, Nicoletta
AU - Ryan, Anthony
AU - Biasi, Fiorella
AU - Vergani, Carlo
AU - Annoni, Giorgio
PY - 1997
Y1 - 1997
N2 - We explored the protective activity of glutathione (GSH) in a rat model of carbon tetrachloride-induced acute liver damage. Histological examination of livers from GSH-pretreated rats revealed minor damage, confirmed by biochemical parameters of liver cell necrosis evaluated both 24 and 48 hr after hepatotoxin delivery. In addition we quantified changes in hepatic steady-state levels of albumin, type I procollagen, transforming growth factor-β1 and tumour necrosis factor-α mRNAs. Even at the molecular level and above all for the albumin gene, it appears that GSH lessens the effect of the hepatotoxin, however the protection of the thiol is restricted to the first 24 hrs and is almost totally exhausted after 48 hr. Since, only 24 hr after CCl4 delivery, GSH abundance determined in erythrocytes and liver is almost equal in the controls and in the GSH injected rats, but significantly higher than in the only intoxicated animals (P <0.05, intraerythrocyte content), we conclude that the thiol pretreatment exerts an effective but transient protection.
AB - We explored the protective activity of glutathione (GSH) in a rat model of carbon tetrachloride-induced acute liver damage. Histological examination of livers from GSH-pretreated rats revealed minor damage, confirmed by biochemical parameters of liver cell necrosis evaluated both 24 and 48 hr after hepatotoxin delivery. In addition we quantified changes in hepatic steady-state levels of albumin, type I procollagen, transforming growth factor-β1 and tumour necrosis factor-α mRNAs. Even at the molecular level and above all for the albumin gene, it appears that GSH lessens the effect of the hepatotoxin, however the protection of the thiol is restricted to the first 24 hrs and is almost totally exhausted after 48 hr. Since, only 24 hr after CCl4 delivery, GSH abundance determined in erythrocytes and liver is almost equal in the controls and in the GSH injected rats, but significantly higher than in the only intoxicated animals (P <0.05, intraerythrocyte content), we conclude that the thiol pretreatment exerts an effective but transient protection.
UR - http://www.scopus.com/inward/record.url?scp=0030747067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030747067&partnerID=8YFLogxK
M3 - Article
C2 - 9353846
AN - SCOPUS:0030747067
VL - 81
SP - 164
EP - 168
JO - Pharmacology and Toxicology
JF - Pharmacology and Toxicology
SN - 0901-9928
IS - 4
ER -