Gluten sensitivity of small intestinal mucosa in vitro: Quantitative assessment of histologic change

P. D. Howdle, G. R. Corazza, A. W. Bullen, M. S. Losowsky

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.

Original languageEnglish
Pages (from-to)442-450
Number of pages9
JournalGastroenterology
Volume80
Issue number3
Publication statusPublished - 1981

Fingerprint

Glutens
Intestinal Mucosa
Enterocytes
Abdomen
Organ Culture Techniques
In Vitro Techniques
Mucous Membrane
Celiac Disease

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Gluten sensitivity of small intestinal mucosa in vitro : Quantitative assessment of histologic change. / Howdle, P. D.; Corazza, G. R.; Bullen, A. W.; Losowsky, M. S.

In: Gastroenterology, Vol. 80, No. 3, 1981, p. 442-450.

Research output: Contribution to journalArticle

Howdle, P. D. ; Corazza, G. R. ; Bullen, A. W. ; Losowsky, M. S. / Gluten sensitivity of small intestinal mucosa in vitro : Quantitative assessment of histologic change. In: Gastroenterology. 1981 ; Vol. 80, No. 3. pp. 442-450.
@article{e8b9fb86fb0849729c3af44b02b64171,
title = "Gluten sensitivity of small intestinal mucosa in vitro: Quantitative assessment of histologic change",
abstract = "In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.",
author = "Howdle, {P. D.} and Corazza, {G. R.} and Bullen, {A. W.} and Losowsky, {M. S.}",
year = "1981",
language = "English",
volume = "80",
pages = "442--450",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Gluten sensitivity of small intestinal mucosa in vitro

T2 - Quantitative assessment of histologic change

AU - Howdle, P. D.

AU - Corazza, G. R.

AU - Bullen, A. W.

AU - Losowsky, M. S.

PY - 1981

Y1 - 1981

N2 - In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.

AB - In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.

UR - http://www.scopus.com/inward/record.url?scp=0019364795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019364795&partnerID=8YFLogxK

M3 - Article

C2 - 7450439

AN - SCOPUS:0019364795

VL - 80

SP - 442

EP - 450

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 3

ER -