Abstract
In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.
| Original language | English |
|---|---|
| Pages (from-to) | 442-450 |
| Number of pages | 9 |
| Journal | Gastroenterology |
| Volume | 80 |
| Issue number | 3 |
| Publication status | Published - 1981 |
Fingerprint
ASJC Scopus subject areas
- Gastroenterology
Cite this
Gluten sensitivity of small intestinal mucosa in vitro : Quantitative assessment of histologic change. / Howdle, P. D.; Corazza, G. R.; Bullen, A. W.; Losowsky, M. S.
In: Gastroenterology, Vol. 80, No. 3, 1981, p. 442-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gluten sensitivity of small intestinal mucosa in vitro
T2 - Quantitative assessment of histologic change
AU - Howdle, P. D.
AU - Corazza, G. R.
AU - Bullen, A. W.
AU - Losowsky, M. S.
PY - 1981
Y1 - 1981
N2 - In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.
AB - In view of the importance to the patient of demonstrating mucosal sensitivity to gluten we have quantitated morphologic change during organ culture of small bowel mucosa by measuring enterocyte height. In 27 normal controls the mean preculture enterocyte height was 28.3 μm ± 1.8 (±1 SD), decreasing to 25.6 μm ± 1.9 and 26.1 μm ± 2.0 after 24 h culture in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but there was no difference between culture with or without gluten. In 9 abnormal controls, the mean preculture enterocyte height was 27.0 μm ± 2.7, decreasing significantly (p <0.05) to 24.5 μn ± 1.1 after 24 h culture without gluten, and to 25.4 μm ± 2.3 with gluten (NS). In 17 untreated celiac patients, the mean preculture enterocyte height was 19.6 μm ± 1.9 increasing significantly to 23.8 μm ± 2.0 (p <0.001) after 24 h culture without gluten but decreasing to 18.7 μm ± 3.1 after 24 h culture with gluten (NS). There was a significant difference between culture with or without gluten (p <0.001). In 21 treated celiac patients, the mean preculture enterocyte height was 28.1 μm ± 1.9 decreasing to 25.4 μm ± 2.0 and 24.2 μm ± 1.9 in the absence and presence of gluten, respectively. Both these decreases were significant (p <0.001), but more importantly there was a significant decrease (p <0.001) after culture with gluten compared with culture without gluten. These data indicate that it is possible to quantify morphologic change during organ culture and to demonstrate gluten-sensitivity not only in untreated, but also in treated celiac mucosa. These studies have important implications as regards the diagnois and further investigation of the etiology of celiac disease.
UR - http://www.scopus.com/inward/record.url?scp=0019364795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019364795&partnerID=8YFLogxK
M3 - Article
C2 - 7450439
AN - SCOPUS:0019364795
VL - 80
SP - 442
EP - 450
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 3
ER -